Toxicity screenings of nanomaterials: challenges due to interference with assay processes and components of classic in vitro tests.

Détails

ID Serval
serval:BIB_A57498A077DD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Toxicity screenings of nanomaterials: challenges due to interference with assay processes and components of classic in vitro tests.
Périodique
Nanotoxicology
Auteur(s)
Guadagnini R., Halamoda Kenzaoui B., Walker L., Pojana G., Magdolenova Z., Bilanicova D., Saunders M., Juillerat-Jeanneret L., Marcomini A., Huk A., Dusinska M., Fjellsbø L.M., Marano F., Boland S.
ISSN
1743-5404 (Electronic)
ISSN-L
1743-5390
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
9
Pages
13-24
Langue
anglais
Notes
Publication types: Journal Article Publication Status: ppublish
Résumé
Given the multiplicity of nanoparticles (NPs), there is a requirement to develop screening strategies to evaluate their toxicity. Within the EU-funded FP7 NanoTEST project, a panel of medically relevant NPs has been used to develop alternative testing strategies of NPs used in medical diagnostics. As conventional toxicity tests cannot necessarily be directly applied to NPs in the same manner as for soluble chemicals and drugs, we determined the extent of interference of NPs with each assay process and components. In this study, we fully characterized the panel of NP suspensions used in this project (poly(lactic-co-glycolic acid)-polyethylene oxide [PLGA-PEO], TiO2, SiO2, and uncoated and oleic-acid coated Fe3O4) and showed that many NP characteristics (composition, size, coatings, and agglomeration) interfere with a range of in vitro cytotoxicity assays (WST-1, MTT, lactate dehydrogenase, neutral red, propidium iodide, (3)H-thymidine incorporation, and cell counting), pro-inflammatory response evaluation (ELISA for GM-CSF, IL-6, and IL-8), and oxidative stress detection (monoBromoBimane, dichlorofluorescein, and NO assays). Interferences were assay specific as well as NP specific. We propose how to integrate and avoid interference with testing systems as a first step of a screening strategy for biomedical NPs.
Pubmed
Web of science
Création de la notice
31/05/2015 9:45
Dernière modification de la notice
20/08/2019 16:10
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