Comparative Analysis of Three Bradyrhizobium diazoefficiens Genomes Show Specific Mutations Acquired during Selection for a Higher Motility Phenotype and Adaption to Laboratory Conditions.

Détails

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Etat: Public
Version: Final published version
Licence: Non spécifiée
ID Serval
serval:BIB_A54BFEC7CED2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Comparative Analysis of Three Bradyrhizobium diazoefficiens Genomes Show Specific Mutations Acquired during Selection for a Higher Motility Phenotype and Adaption to Laboratory Conditions.
Périodique
Microbiology spectrum
Auteur⸱e⸱s
Lozano M.J., Redondo-Nieto M., Garrido-Sanz D., Mongiardini E., Quelas J.I., Mengucci F., Dardis C., Lodeiro A., Althabegoiti M.J.
ISSN
2165-0497 (Electronic)
ISSN-L
2165-0497
Statut éditorial
Publié
Date de publication
22/12/2021
Peer-reviewed
Oui
Volume
9
Numéro
3
Pages
e0056921
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Microbial genomes are being extensively studied using next-generation sequencing technologies in order to understand the changes that occur under different selection regimes. In this work, the number and type of mutations that have occurred in three Bradyrhizobium diazoefficiens USDA 110 <sup>T</sup> strains under laboratory conditions and during selection for a more motile phenotypic variant were analyzed. Most of the mutations found in both processes consisted of single nucleotide polymorphisms, single nucleotide deletions or insertions. In the case of adaptation to laboratory conditions, half of the changes occurred within intergenic regions, and around 80% were insertions. When the more motile phenotypic variant was evaluated, eight single nucleotide polymorphisms and an 11-bp deletion were found, although none of them was directly related to known motility or chemotaxis genes. Two mutants were constructed to evaluate the 11-bp deletion affecting the alpha subunit of 2-oxoacid:acceptor oxidoreductase (AAV28_RS30705-blr6743). The results showed that this single deletion was not responsible for the enhanced motility phenotype. IMPORTANCE The genetic and genomic changes that occur under laboratory conditions in Bradyrhizobium diazoefficiens genomes remain poorly studied. Only a few genome sequences of this important nitrogen-fixing species are available, and there are no genome-wide comparative analyses of related strains. In the present work, we sequenced and compared the genomes of strains derived from a parent strain, B. diazoefficiens USDA 110, that has undergone processes of repeated culture in the laboratory environment, or phenotypic selection toward antibiotic resistance and enhanced motility. Our results represent the first analysis in B. diazoefficiens that provides insights into the specific mutations that are acquired during these processes.
Mots-clé
Adaptation, Biological, Bradyrhizobium/cytology, Bradyrhizobium/genetics, Bradyrhizobium/physiology, Genome, Bacterial, Genomics, Mutation, Phylogeny, Polymorphism, Single Nucleotide, Symbiosis, comparative genomics, laboratory adaptation, single nucleotide polymorphism, swimming motility
Pubmed
Web of science
Open Access
Oui
Création de la notice
18/03/2022 18:40
Dernière modification de la notice
25/01/2024 7:41
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