NineTeen Complex-subunit Salsa is required for efficient splicing of a subset of introns and dorsal-ventral patterning.

Détails

ID Serval
serval:BIB_A51FB6FD539B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
NineTeen Complex-subunit Salsa is required for efficient splicing of a subset of introns and dorsal-ventral patterning.
Périodique
RNA
Auteur(s)
Rathore O.S., Silva R.D., Ascensão-Ferreira M., Matos R., Carvalho C., Marques B., Tiago M.N., Prudêncio P., Andrade R.P., Roignant J.Y., Barbosa-Morais N.L., Martinho R.G.
ISSN
1469-9001 (Electronic)
ISSN-L
1355-8382
Statut éditorial
Publié
Date de publication
12/2020
Peer-reviewed
Oui
Volume
26
Numéro
12
Pages
1935-1956
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
The NineTeen Complex (NTC), also known as pre-mRNA-processing factor 19 (Prp19) complex, regulates distinct spliceosome conformational changes necessary for splicing. During Drosophila midblastula transition, splicing is particularly sensitive to mutations in NTC-subunit Fandango, which suggests differential requirements of NTC during development. We show that NTC-subunit Salsa, the Drosophila ortholog of human RNA helicase Aquarius, is rate-limiting for splicing of a subset of small first introns during oogenesis, including the first intron of gurken Germline depletion of Salsa and splice site mutations within gurken first intron impair both adult female fertility and oocyte dorsal-ventral patterning, due to an abnormal expression of Gurken. Supporting causality, the fertility and dorsal-ventral patterning defects observed after Salsa depletion could be suppressed by the expression of a gurken construct without its first intron. Altogether, our results suggest that one of the key rate-limiting functions of Salsa during oogenesis is to ensure the correct expression and efficient splicing of the first intron of gurken mRNA. Retention of gurken first intron compromises the function of this gene most likely because it undermines the correct structure and function of the transcript 5'UTR.
Mots-clé
Dorsal-ventral patterning, Drosophila, Female fertility, Gurken, Splicing, dorsal–ventral patterning, female fertility, splicing
Pubmed
Web of science
Création de la notice
28/09/2020 16:59
Dernière modification de la notice
23/12/2020 7:24
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