Mutations in the hormone regulatory element of mouse mammary tumor virus differentially affect the response to progestins, androgens, and glucocorticoids

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Etat: Public
Version: de l'auteur⸱e
ID Serval
serval:BIB_A4B3C0DD52B0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Mutations in the hormone regulatory element of mouse mammary tumor virus differentially affect the response to progestins, androgens, and glucocorticoids
Périodique
Molecular and Cellular Biology
Auteur⸱e⸱s
Gowland P. L., Buetti E.
ISSN
0270-7306 (Print)
Statut éditorial
Publié
Date de publication
09/1989
Volume
9
Numéro
9
Pages
3999-4008
Notes
Journal Article Research Support, Non-U.S. Gov't --- Old month value: Sep
Résumé
Transcription of the mouse mammary tumor virus DNA is known to be induced by several steroid hormones. Using chimeric MMTV plasmids containing mutations within the hormone regulatory element, we have previously studied the regions required for the glucocorticoid response in mouse fibroblasts. Here we report the characterization of elements essential for the stimulation by progestins and androgens as compared with glucocorticoids. The same set of mutant plasmids was transfected into the human mammary tumor cell line T47D, and the specific transcripts were analyzed by an S1 nuclease protection assay. Androgen-mediated stimulation, although weak, showed an extended sensitivity to mutations, with a slight preference for the proximal region. The results with progestin suggest that sequences within all the described sites protected by the receptor in vitro are required and that the promoter-proximal region (-128 to -78 from the RNA start site) is more important than the distal one (-190 to -160). Moreover, a binding site for nuclear factor I was not required for the progestin response, whereas it was required for glucocorticoids. Thus, the various steroid receptors play a role in the differential regulation of mouse mammary tumor virus transcription by recognizing distinct sequence differences in the hormone regulatory element and interacting with different factors bound to the promoter.
Mots-clé
Androgens/pharmacology Animals Base Sequence DNA, Viral/genetics Genes, Viral Glucocorticoids/pharmacology Hormones/*pharmacology Humans Mammary Tumor Virus, Mouse/drug effects/*genetics Mice Molecular Sequence Data Mutation Progestins/pharmacology Promoter Regions (Genetics)/drug effects Repetitive Sequences, Nucleic Acid Transcription, Genetic/drug effects
Pubmed
Web of science
Création de la notice
25/01/2008 15:25
Dernière modification de la notice
20/08/2019 16:10
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