Delineation of PLA2 epitopes using short or long overlapping synthetic peptides: interest for specific immunotherapy

Détails

ID Serval
serval:BIB_A4B24540D65A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Delineation of PLA2 epitopes using short or long overlapping synthetic peptides: interest for specific immunotherapy
Périodique
Clinical and Experimental Allergy
Auteur⸱e⸱s
Kammerer  R., Kettner  A., Chvatchko  Y., Dufour  N., Tiercy  J. M., Corradin  G., Spertini  F.
ISSN
0954-7894 (Print)
Statut éditorial
Publié
Date de publication
09/1997
Volume
27
Numéro
9
Pages
1016-26
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep
Résumé
BACKGROUND: Venom immunotherapy is definitely indicated in severe systemic anaphylactic reactions to bee stings, but is not devoided of risks of anaphylaxis. Safer methods of immunotherapy need to be developed. OBJECTIVE: To delineate phospholipase A2 T-cell epitopes using short 15mer vs long 40-60mer overlapping peptides, and to approach the potential interest of a venom immunotherapy based on the use of long peptides (1-60, 51-99, 90-134) mapping the whole phospholipase A2 molecule vs a restricted number of immunodominant epitopes. METHODS: Proliferation of a CD8+ T cell depleted peripheral blood mononuclear cell fraction and short-term T-cell lines from unselected bee venom hypersensitive patients in response to phospholipase A2 synthetic peptides. RESULTS: Whereas T-cell proliferation to 15mer overlapping peptides was weak, T-cell response to long overlapping peptides was in contrast vigorous in all patients, mostly directed to C-terminal peptide 90-134. Our results did not support the concept of rare dominant T-cell epitopes, and disclosed T-cell responses to multiple epitopes in several patients. No significant IgE-binding to long overlapping peptides was detected except in one patient against peptide 90-134. CONCLUSION: 15mer peptides might not be sensitive enough to fully delineate all potential T-cell epitopes scattered along the allergen. Since they do not bind IgE in vitro or only weakly, and taking into account a T-cell response frequently directed to multiple epitopes, long overlapping peptides may represent ideal tools for immunotherapy.
Mots-clé
Bee Venoms/*immunology Cell Line *Desensitization, Immunologic Epitope Mapping Epitopes, T-Lymphocyte/immunology Humans Hypersensitivity, Immediate/*therapy Immunoblotting Immunoglobulin E/metabolism Leukocytes, Mononuclear Peptides/immunology Phospholipases A/*immunology T-Lymphocytes/immunology
Pubmed
Web of science
Création de la notice
24/01/2008 14:55
Dernière modification de la notice
20/08/2019 15:10
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