Constitutive expression of inducible nitric oxide synthase in human bronchial epithelial cells induces c-fos and stimulates the cGMP pathway.

Détails

ID Serval
serval:BIB_A4AEA7BCB214
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Constitutive expression of inducible nitric oxide synthase in human bronchial epithelial cells induces c-fos and stimulates the cGMP pathway.
Périodique
American Journal of Respiratory Cell and Molecular Biology
Auteur⸱e⸱s
Felley-Bosco E., Ambs S., Lowenstein C.J., Keefer L.K., Harris C.C.
ISSN
1044-1549 (Print)
ISSN-L
1044-1549
Statut éditorial
Publié
Date de publication
1994
Volume
11
Numéro
2
Pages
159-164
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
Two major roles have been defined for nitric oxide (NO): cell-cell communication mediated by the stimulation of cyclic guanosine 3',5'-monophosphate (cGMP) synthesis and cytotoxicity by direct or indirect interaction of the free radical NO with cellular targets. Thus, pathologic states might result from an alteration of NO pathways, e.g., by deregulated activity of NO synthase. To investigate this hypothesis, we introduced the murine-inducible NO synthase (iNOS) sequence into immortalized human bronchial epithelial cells (BEAS-2B). iNOS activity, measured by conversion of [14C]arginine to [14C]citrulline in the presence of 1 mM EGTA, was higher than 100 pmol/min/mg protein in early passages of iNOS-transfected cells but decreased with cell subculturing. No iNOS activity could be detected in control vector-transfected cells. NO stimulated cGMP production in iNOS-transfected cells, and this effect was inhibited by the iNOS inhibitor NG-monomethyl-L-arginine. In addition, NO production induced c-fos expression and did not interfere with clonal cell growth. These results suggest that BEAS-2B cells constitute a suitable model to study the consequences of iNOS activity on signal transduction pathways in bronchial epithelium.
Mots-clé
Amino Acid Oxidoreductases/biosynthesis, Animals, Antigens, Polyomavirus Transforming/biosynthesis, Arginine/metabolism, Base Sequence, Blotting, Northern, Bronchi/metabolism, Carbon Radioisotopes, Cell Division, Cell Line, Transformed, Citrulline/metabolism, Cyclic GMP/metabolism, DNA Primers, Enzyme Induction, Epithelium/metabolism, Gene Expression, Genes, fos, Humans, Kinetics, Mice, Molecular Sequence Data, Nitric Oxide Synthase, Polymerase Chain Reaction, Proto-Oncogene Proteins c-fos/biosynthesis, Simian virus 40/genetics, Transfection
Pubmed
Web of science
Création de la notice
14/01/2016 19:00
Dernière modification de la notice
20/08/2019 15:10
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