Evaluation and usefulness of reverse dot blot DNA-PolyMarker typing in forensic case work.
Détails
ID Serval
serval:BIB_A47182DA753A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Evaluation and usefulness of reverse dot blot DNA-PolyMarker typing in forensic case work.
Périodique
Forensic Science International
ISSN
0379-0738
Statut éditorial
Publié
Date de publication
1996
Volume
81
Numéro
1
Pages
61-72
Langue
anglais
Résumé
Experiments were performed to evaluate the Amplitype PolyMarker DNA typing system for application to forensic casework. DNA extraction using chelex was compared with phenol-chloroform extraction for various biological materials including postmortem blood, blood samples used for alcohol quantification, fresh urine, envelopes and cigarette butts. Different amounts of genomic DNA were amplified to test the sensitivity of the Amplitype PM. Mixed samples of two different bloods were typed to determine the dilution at which mixtures could be detected. Different storage conditions were evaluated using urine samples. Postmortem blood samples were typed during 4 months to determine the effects of natural degradation. A population sample of 105 unrelated individuals from South-West Switzerland was analyzed and the genotype frequencies were compared with those reported by others. Finally, practical usefulness of the Amplitype PM system is illustrated by analysing casework samples. The results of this validation proved the great usefulness and sensitivity of the Amplitype PM system using the appropriate extraction and typing method. However, mixed samples had to be interpreted with caution owing to the possibility of non-specific alleles with stored material such as urine and postmortem blood.
Mots-clé
Alleles, DNA, Gene Frequency, Genotype, Humans, Polymerase Chain Reaction, Sensitivity and Specificity, Switzerland
Pubmed
Web of science
Création de la notice
08/02/2008 17:44
Dernière modification de la notice
20/08/2019 15:09