Physicochemical and biological characterization of single-walled and double-walled carbon nanotubes in biological media

Détails

ID Serval
serval:BIB_A4586CB9DD33
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Physicochemical and biological characterization of single-walled and double-walled carbon nanotubes in biological media
Périodique
Journal of Hazardous Materials
Auteur⸱e⸱s
Liu W.T., Bien M.Y., Chuang K.J., Chang T.Y., Jones T., BéruBé K., Lalev G., Tsai D.H., Chuang H.C., Cheng T.J.
Collaborateur⸱rice⸱s
Taiwan CardioPulmonary Research (T-CPR) Group
ISSN
1873-3336 (Electronic)
ISSN-L
0304-3894
Statut éditorial
Publié
Date de publication
09/2014
Peer-reviewed
Oui
Volume
280
Pages
216-225
Langue
anglais
Notes
IUMSP2014/09
Résumé
To study the toxicity of nanoparticles under relevant conditions, it is important to reproducibly disperse nanoparticles in biological media in in vitro and in vivo studies. Here, single-walled nanotubes (SWNTs) and double-walled nanotubes (DWNTs) were physicochemically and biologically characterized when dispersed in phosphate-buffered saline (PBS) and bovine serum albumin (BSA). BSA-SWNT/DWNT interaction resulted in a reduction of aggregation and an increase in particle stabilization. Based on the protein sequence coverage and protein binding results, DWNTs exhibited higher protein binding than SWNTs. SWNT and DWNT suspensions in the presence of BSA increased interleukin-6 (IL-6) levels and reduced tumor necrosis factor-alpha (TNF-α) levels in A549 cells as compared to corresponding samples in the absence of BSA. We next determined the effects of SWNTs and DWNTs on pulmonary protein modification using bronchoalveolar lavage fluid (BALF) as a surrogate collected form BALB/c mice. The BALF proteins bound to SWNTs (13 proteins) and DWNTs (11 proteins), suggesting that these proteins were associated with blood coagulation pathways. Lastly, we demonstrated the importance of physicochemical and biological alterations of SWNTs and DWNTs when dispersed in biological media, since protein binding may result in the misinterpretation of in vitro results and the activation of protein-regulated biological responses.
Mots-clé
Carbon nanotube, Inflammation, Nanomaterials, Nanotoxicology, Proteomics
Pubmed
Web of science
Création de la notice
24/09/2014 8:27
Dernière modification de la notice
20/08/2019 15:09
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