Interspecies Chimerism with Mammalian Pluripotent Stem Cells.

Détails

ID Serval
serval:BIB_A42FFFB27730
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Interspecies Chimerism with Mammalian Pluripotent Stem Cells.
Périodique
Cell
Auteur(s)
Wu J., Platero-Luengo A., Sakurai M., Sugawara A., Gil M.A., Yamauchi T., Suzuki K., Bogliotti Y.S., Cuello C., Morales Valencia M., Okumura D., Luo J., Vilariño M., Parrilla I., Soto D.A., Martinez C.A., Hishida T., Sánchez-Bautista S., Martinez-Martinez M.L., Wang H., Nohalez A., Aizawa E., Martinez-Redondo P., Ocampo A., Reddy P., Roca J., Maga E.A., Esteban C.R., Berggren W.T., Nuñez Delicado E., Lajara J., Guillen I., Guillen P., Campistol J.M., Martinez E.A., Ross P.J., Izpisua Belmonte J.C.
ISSN
1097-4172 (Electronic)
ISSN-L
0092-8674
Statut éditorial
Publié
Date de publication
26/01/2017
Peer-reviewed
Oui
Volume
168
Numéro
3
Pages
473-486.e15
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Interspecies blastocyst complementation enables organ-specific enrichment of xenogenic pluripotent stem cell (PSC) derivatives. Here, we establish a versatile blastocyst complementation platform based on CRISPR-Cas9-mediated zygote genome editing and show enrichment of rat PSC-derivatives in several tissues of gene-edited organogenesis-disabled mice. Besides gaining insights into species evolution, embryogenesis, and human disease, interspecies blastocyst complementation might allow human organ generation in animals whose organ size, anatomy, and physiology are closer to humans. To date, however, whether human PSCs (hPSCs) can contribute to chimera formation in non-rodent species remains unknown. We systematically evaluate the chimeric competency of several types of hPSCs using a more diversified clade of mammals, the ungulates. We find that naïve hPSCs robustly engraft in both pig and cattle pre-implantation blastocysts but show limited contribution to post-implantation pig embryos. Instead, an intermediate hPSC type exhibits higher degree of chimerism and is able to generate differentiated progenies in post-implantation pig embryos.
Mots-clé
Animals, Blastocyst, CRISPR-Cas Systems, Cattle, Chimerism, Embryo, Mammalian/cytology, Female, Gene Editing, Humans, Male, Mammals/classification, Mammals/embryology, Mice, Mice, Inbred C57BL, Mice, Inbred ICR, Pluripotent Stem Cells, Rats, Rats, Sprague-Dawley, Sus scrofa, CRISPR-Cas9, human naïve pluripotent stem cells, human-cattle chimeric embryo, human-pig chimeric embryo, interspecies blastocyst complementation, interspecies chimera, organ and tissue generation, pluripotent stem cells, zygote genome editing
Pubmed
Web of science
Création de la notice
14/08/2018 10:28
Dernière modification de la notice
20/08/2019 16:09
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