Saccamoeba lacustris, sp. nov. (Amoebozoa: Lobosea: Hartmannellidae), a new lobose amoeba, parasitized by the novel chlamydia 'Candidatus Metachlamydia lacustris' (Chlamydiae: Parachlamydiaceae).

Détails

ID Serval
serval:BIB_A420863EC33C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Saccamoeba lacustris, sp. nov. (Amoebozoa: Lobosea: Hartmannellidae), a new lobose amoeba, parasitized by the novel chlamydia 'Candidatus Metachlamydia lacustris' (Chlamydiae: Parachlamydiaceae).
Périodique
European Journal of Protistology
Auteur(s)
Corsaro Daniele, Michel Rolf, Walochnik Julia, Mueller Karl-Dieter, Greub Gilbert
ISSN
1618-0429[electronic], 0932-4739[linking]
Statut éditorial
Publié
Date de publication
2010
Volume
46
Numéro
2
Pages
86-95
Langue
anglais
Résumé
An amoeba isolated from an aquatic biotope, identified morphologically as Saccamoeba limax, was found harbouring mutualistic rod-shaped gram-negative bacteria. During their cultivation on agar plates, a coinfection also by lysis-inducing chlamydia-like organisms was found in some subpopulations of that amoeba. .Here we provide a molecular-based identification of both the amoeba host and the two bacterial endosymbionts. Analysis of the 18S rRNA gene revealed that this strain is the sister-group to Glaeseria, for which we proposed the name Saccamoeba lacustris. The rod-shaped endosymbiont was identified as a member of Variovorax paradoxus group (Comamonadaceae, Beta-Proteobacteria). No growth on bacteriological agars was recorded, hence this symbiont might be strictly intracellular. The chlamydia-like parasite was unable to infect Acanthamoeba and other amoebae in coculture, showing high host specificity. Phylogenetic analysis based on the 16S rDNA indicated that it is a new member of the family Parachlamydiaceae (order Chlamydiales), for which we proposed the name 'Candidatus Metachlamydia lacustris'.
Mots-clé
Amoebozoa, Saccamoeba, Metachlamydia, Variovorax, Endosymbiont, Free-Living Amebas, Bacterial Endosymbionts, Naegleria-Strain, Acanthamoeba, Diversity, Identification, Environment, Organisms, Sequence, Cysts
Pubmed
Web of science
Création de la notice
23/06/2010 14:06
Dernière modification de la notice
20/08/2019 16:09
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