Vesicular transport of d-serine in astrocytes

Détails

ID Serval
serval:BIB_A3ACC4C4E478
Type
Actes de conférence (partie): contribution originale à la littérature scientifique, publiée à l'occasion de conférences scientifiques, dans un ouvrage de compte-rendu (proceedings), ou dans l'édition spéciale d'un journal reconnu (conference proceedings).
Sous-type
Abstract (résumé de présentation): article court qui reprend les éléments essentiels présentés à l'occasion d'une conférence scientifique dans un poster ou lors d'une intervention orale.
Collection
Publications
Institution
Titre
Vesicular transport of d-serine in astrocytes
Titre de la conférence
10th European meeting on Glial Cells in Health and Disease
Auteur⸱e⸱s
Mothet J. P., Shi T., Puyal J., Klingauf J., Sweedler J., Jahn R., Martineau M.
Adresse
Prague, Czech Republic, September 13-17, 2011
ISBN
0894-1491
Statut éditorial
Publié
Date de publication
2011
Volume
59
Série
GLIA
Pages
S34-S35
Langue
anglais
Notes
Publication type : Meeting Abstract
Résumé
The concept of tripartite synapse suggests that astrocytes make up a functional synapse with pre- and postsynaptic neuronal elements to modulate synaptic transmission through the regulated release of neuromodulators called gliotransmitters. Release of gliotransmitters such as glutamate or D-serine has been shown to depend on Ca21-dependent exocytosis. However, the origin (cytosolic versus vesicular) of the released gliotransmitter is still a matter of debate. The existence of Ca21-regulated exocytosis in astrocytes has been questioned mostly because the nature of secretory organelles which are loaded with gliotransmitters is unknown. Here we show the existence of a population of vesicles that uptakes and stores glutamate and D-serine in astrocytes which are present in situ. Immunoisolated glial organelles expressing synaptobrevin 2 (Sb2) display morphological and biochemical features very similar to synaptic vesicles. We demonstrate that these organelles not only contain and uptake glutamate but also display a glia-specific transport activity for D-serine. Furthermore, we report that the uptake of D-serine is energized by a H1-ATPase present on the immunoisolated vesicles and that cytosolic chloride ions modulate the uptake of D-serine. Finally, we show that serine racemase (SR), the synthesizing enzyme for D-serine, is anchored to the membrane of glial organelles allowing a local and efficient concentration of the gliotransmitter to be transported. We conclude that vesicles in astrocytes do exist with the goal to store and release D-serine, glutamate and most likely other neuromodulators.
Mots-clé
D-serine, astrocytes, vesicular transporter,
Web of science
Création de la notice
23/09/2011 11:06
Dernière modification de la notice
20/08/2019 15:09
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