Push-pull cannula for localized application of drugs and sampling of medium, combined with electrophysiological recordings in an interface slice chamber.

Détails

ID Serval
serval:BIB_A2E2FCA1F5B8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Push-pull cannula for localized application of drugs and sampling of medium, combined with electrophysiological recordings in an interface slice chamber.
Périodique
Journal of Neuroscience Methods
Auteur⸱e⸱s
West D.C., Thomson A.M., Do K.Q.
ISSN
0165-0270 (Print)
ISSN-L
0165-0270
Statut éditorial
Publié
Date de publication
1992
Peer-reviewed
Oui
Volume
43
Numéro
1
Pages
35-42
Langue
anglais
Notes
Publication types: In Vitro ; Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
These experiments combined electrophysiological recordings from hippocampal slices with application of drugs to and sampling of extracellular fluid from a restricted region of the slice using a push-pull cannula placed under the slice in an interface chamber. Stable and apparently normal extracellular and intracellular recordings could be obtained directly over the tip of the cannula and solutions changed without disturbing the recording. Relatively rapid effects (1-5 min) were observed when TTX, CNQX, or medium containing 50 mM K+ were applied via the cannula and recovery from these effects was achieved. In addition, effects were restricted to the immediate vicinity of the cannula; neurones recorded several hundred micrometers away were apparently unaffected. Samples of extracellular fluid obtained as minute fractions during the application of high K+ containing medium contained higher concentrations of GABA, aspartate and glutamate than control fractions but the same levels of other amino acids, e.g., isoleucine and leucine. With appropriate design of push-pull cannula and recording chamber, therefore, stable electrophysiological recordings can be combined with localized extracellular fluid sampling and rapid and localized application of test solutions in an interface slice chamber.
Mots-clé
6-Cyano-7-nitroquinoxaline-2,3-dione, Amino Acids/analysis, Amino Acids/metabolism, Animals, Catheterization/instrumentation, Chromatography, High Pressure Liquid, Culture Media, Electrophysiology, Extracellular Space/physiology, Hippocampus/anatomy & histology, Hippocampus/physiology, Male, Pharmaceutical Preparations/administration & dosage, Potassium/pharmacology, Quinoxalines/pharmacology, Rats, Sodium/pharmacology, Tetrodotoxin/pharmacology
Pubmed
Web of science
Création de la notice
07/03/2014 9:47
Dernière modification de la notice
20/08/2019 16:08
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