A reversible gene trap collection empowers haploid genetics in human cells.

Détails

ID Serval
serval:BIB_A2D837180513
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A reversible gene trap collection empowers haploid genetics in human cells.
Périodique
Nature methods
Auteur(s)
Bürckstümmer T., Banning C., Hainzl P., Schobesberger R., Kerzendorfer C., Pauler F.M., Chen D., Them N., Schischlik F., Rebsamen M., Smida M., Fece de la Cruz F., Lapao A., Liszt M., Eizinger B., Guenzl P.M., Blomen V.A., Konopka T., Gapp B., Parapatics K., Maier B., Stöckl J., Fischl W., Salic S., Taba Casari M.R., Knapp S., Bennett K.L., Bock C., Colinge J., Kralovics R., Ammerer G., Casari G., Brummelkamp T.R., Superti-Furga G., Nijman S.M.
ISSN
1548-7105 (Electronic)
ISSN-L
1548-7091
Statut éditorial
Publié
Date de publication
10/2013
Peer-reviewed
Oui
Volume
10
Numéro
10
Pages
965-971
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Knockout collections are invaluable tools for studying model organisms such as yeast. However, there are no large-scale knockout collections of human cells. Using gene-trap mutagenesis in near-haploid human cells, we established a platform to generate and isolate individual 'gene-trapped cells' and used it to prepare a collection of human cell lines carrying single gene-trap insertions. In most cases, the insertion can be reversed. This growing library covers 3,396 genes, one-third of the expressed genome, is DNA-barcoded and allows systematic screens for a wide variety of cellular phenotypes. We examined cellular responses to TNF-α, TGF-β, IFN-γ and TNF-related apoptosis-inducing ligand (TRAIL), to illustrate the value of this unique collection of isogenic human cell lines.
Mots-clé
Cell Line, Tumor, Gene Library, Genome, Human, Haploidy, Humans, Molecular Sequence Data, Mutagenesis, Insertional/methods, Reverse Genetics/methods
Pubmed
Web of science
Création de la notice
11/08/2020 17:06
Dernière modification de la notice
13/08/2020 6:26
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