A reversible gene trap collection empowers haploid genetics in human cells.
Détails
ID Serval
serval:BIB_A2D837180513
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
A reversible gene trap collection empowers haploid genetics in human cells.
Périodique
Nature methods
ISSN
1548-7105 (Electronic)
ISSN-L
1548-7091
Statut éditorial
Publié
Date de publication
10/2013
Peer-reviewed
Oui
Volume
10
Numéro
10
Pages
965-971
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
Knockout collections are invaluable tools for studying model organisms such as yeast. However, there are no large-scale knockout collections of human cells. Using gene-trap mutagenesis in near-haploid human cells, we established a platform to generate and isolate individual 'gene-trapped cells' and used it to prepare a collection of human cell lines carrying single gene-trap insertions. In most cases, the insertion can be reversed. This growing library covers 3,396 genes, one-third of the expressed genome, is DNA-barcoded and allows systematic screens for a wide variety of cellular phenotypes. We examined cellular responses to TNF-α, TGF-β, IFN-γ and TNF-related apoptosis-inducing ligand (TRAIL), to illustrate the value of this unique collection of isogenic human cell lines.
Mots-clé
Cell Line, Tumor, Gene Library, Genome, Human, Haploidy, Humans, Molecular Sequence Data, Mutagenesis, Insertional/methods, Reverse Genetics/methods
Pubmed
Web of science
Création de la notice
11/08/2020 17:06
Dernière modification de la notice
13/08/2020 6:26