Use of transgenic mice for the characterization of human alpha 1-acid glycoprotein (orosomucoid) variants.

Détails

ID Serval
serval:BIB_A2B706911F07
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Use of transgenic mice for the characterization of human alpha 1-acid glycoprotein (orosomucoid) variants.
Périodique
Human Genetics
Auteur(s)
Tomei L., Eap C.B., Baumann P., Dente L.
ISSN
0340-6717 (Print)
ISSN-L
0340-6717
Statut éditorial
Publié
Date de publication
1989
Peer-reviewed
Oui
Volume
84
Numéro
1
Pages
89-91
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
Sera from transgenic mice (TM) carrying human genes of alpha 1-acid glycoprotein (orosomucoid or ORM) have been analyzed by isoelectrofocusing and subsequent immunoblotting with antihuman ORM antibodies. With this technique it is possible to reveal selectively the human protein secreted in the TM sera. Orosomucoid bands present in TM sera have been compared with those of the most common human ORM phenotypes to correlate the products of specific genes to previously identified genetic variants. In this paper, we report the identification of the genes encoding for variants ORM1 F1 and ORM2 A, which are genes AGP-A and AGP-B/B' respectively. The nucleotide sequences of these genes are known; therefore a direct correlation between variants and specific amino acid sequences can be established.
Mots-clé
Animals, Genes, Genetic Variation, Humans, Lipopolysaccharides/pharmacology, Mice, Mice, Transgenic, Orosomucoid/biosynthesis, Orosomucoid/genetics, Phenotype
Pubmed
Web of science
Création de la notice
01/03/2013 11:04
Dernière modification de la notice
20/08/2019 16:08
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