Using genetically encoded fluorescent reporters to image lipid signalling in living plants.

Détails

ID Serval
serval:BIB_A1B183684A19
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Institution
Titre
Using genetically encoded fluorescent reporters to image lipid signalling in living plants.
Périodique
Methods In Molecular Biology (clifton, N.j.)
Auteur(s)
Vermeer J.E., Munnik T.
ISSN
1940-6029 (Electronic)
ISSN-L
1064-3745
Statut éditorial
Publié
Date de publication
2013
Peer-reviewed
Oui
Volume
1009
Pages
283-289
Langue
anglais
Notes
Publication types: Journal ArticlePublication Status: ppublish
Résumé
The discovery of the green fluorescent protein has revolutionized cell biology as it allowed researchers to visualize dynamic processes in living cells. The fusion of fluorescent protein variants with lipid binding domains that bind to specific phospholipids have been very instrumental in investigating the role of these molecules in living plants. Here, we describe the use of these reporters to image lipids in living Arabidopsis seedlings using fluorescence microscopy.
Mots-clé
Arabidopsis/metabolism, Genes, Reporter/genetics, Green Fluorescent Proteins/genetics, Lipid Metabolism, Microscopy, Confocal, Molecular Imaging/methods, Seedlings, Signal Transduction
Pubmed
Création de la notice
18/10/2016 14:53
Dernière modification de la notice
20/08/2019 16:07
Données d'usage