Immunochemical evidence for a transmembrane orientation of both the (Na+, K+)-ATPase subunits

Détails

ID Serval
serval:BIB_A174A95A3F7A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Immunochemical evidence for a transmembrane orientation of both the (Na+, K+)-ATPase subunits
Périodique
Biochemistry
Auteur⸱e⸱s
Girardet  M., Geering  K., Frantes  J. M., Geser  D., Rossier  B. C., Kraehenbuhl  J. P., Bron  C.
ISSN
0006-2960
Statut éditorial
Publié
Date de publication
11/1981
Peer-reviewed
Oui
Volume
20
Numéro
23
Pages
6684-91
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov 10
Résumé
Antibodies were raised against the large catalytic subunit (apparent Mr 96000) and the glycoprotein (apparent Mr 60000) of the sodium- and potassium-dependent adenosine triphosphatase [(Na+, K+)-ATPase] from Bufo marinus. The specificity of each antiserum was assessed by two-dimensional immunoelectrophoresis using toad kidney microsomes or the purified holoenzyme as a source of antigen and by indirect immunoprecipitation of detergent-solubilized (Na+, K+)-ATPase subunits from radioiodinated or biosynthetically labeled kidney holoenzyme, microsomes, or postnuclear supernatant. The anticatalytic subunit serum reacted exclusively with a 96000-dalton protein. The antiserum to the glycoprotein was rendered specific to this subunit by absorption with purified catalytic subunit. The two antisera were agglutinating and lytic in the presence of complement when toad erythrocytes were used as targets, indicating that antigenic determinants of both subunits were exposed on the cell surface. The specific reactivities with surface-exposed antigenic determinants of both subunits could be absorbed with toad red blood cells. Such absorbed antisera still reacted with detergent-treated or untreated kidney microsomes, revealing the presence of cytoplasmic and/or intramembranous antigenic sites. Our immunochemical data demonstrate that the glycoprotein subunit of (Na+, K+)-ATPase spans the lipid bilayer and confirm the transmembrane orientation of the catalytic subunit postulated from functional studies.
Mots-clé
Animals Antigen-Antibody Complex Bufo marinus Cytotoxicity, Immunologic Female Freeze Fracturing Hemagglutination Immune Sera Intracellular Membranes/*enzymology Kidney/*enzymology/ultrastructure Macromolecular Substances Male Microscopy, Electron Microsomes/enzymology Molecular Weight Na(+)-K(+)-Exchanging ATPase/immunology/*metabolism
Pubmed
Web of science
Création de la notice
24/01/2008 14:01
Dernière modification de la notice
20/08/2019 16:07
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