Oxygen consumption in collagenase-liberated rat adipocytes in relation to cell size and age.

Détails

ID Serval
serval:BIB_A160F720FC9A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Oxygen consumption in collagenase-liberated rat adipocytes in relation to cell size and age.
Périodique
Metabolism
Auteur⸱e⸱s
Hallgren P., Raddatz E., Bergh C.H., Kucera P., Sjöström L.
ISSN
0026-0495 (Print)
ISSN-L
0026-0495
Statut éditorial
Publié
Date de publication
1984
Volume
33
Numéro
10
Pages
897-900
Langue
anglais
Résumé
Oxygen consumption of collagenase-liberated rat adipocytes was measured by two different techniques: a microspectrophotometric method using hemoglobin as indicator of respiration and a technique using the oxygen electrode. These two completely different techniques gave similar values for oxygen consumption. With the spectrophotometric method, the oxygen consumption of single fat cells was determined. A close positive correlation (r = greater than 0.90) between oxygen consumption and fat cell size was observed in each tissue examined. With the oxygen electrode technique, oxygen consumption of adipocyte suspensions from young (40 days, 180 g) and old (90 days, 480 g) rats was examined. Fat cells of the suspensions were separated into classes of different size by a flotation technique. A significant positive correlation between fat cell size and oxygen consumption was observed in both young (r = 0.88) and old (r = 0.95) rats. However, the slope was much steeper in young rats. At a cell weight of 0.1 microgram the oxygen consumption was 0.364 and 0.086 microL O2/10(6) cells/min-1 in young and old rats, respectively. In the literature, a number of separate metabolic pathways have been found to be related positively to fat cell size and negatively to age. We conclude that these scattered metabolic observations are in agreement with integrated data on energy expenditure as evaluated from oxygen consumption. Estimations of the energy expenditure of adipose tissue indicates that this tissue is responsible for about 1% and 0.5% of the total energy expenditure in young and old rats, respectively.
Mots-clé
Adipose Tissue/cytology, Adipose Tissue/metabolism, Aging, Animals, Cell Separation/methods, Electrodes, Male, Microbial Collagenase, Oxygen Consumption, Rats, Rats, Inbred Strains, Spectrophotometry/methods
Pubmed
Web of science
Création de la notice
24/01/2008 14:19
Dernière modification de la notice
20/08/2019 16:07
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