Effect of mature lymphocytes and lymphotoxin on the development of the follicle-associated epithelium and M cells in mouse Peyer's patches

Détails

ID Serval
serval:BIB_A15470A99BF9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Effect of mature lymphocytes and lymphotoxin on the development of the follicle-associated epithelium and M cells in mouse Peyer's patches
Périodique
Gastroenterology
Auteur(s)
Debard  N., Sierro  F., Browning  J., Kraehenbuhl  J. P.
ISSN
0016-5085 (Print)
Statut éditorial
Publié
Date de publication
04/2001
Volume
120
Numéro
5
Pages
1173-82
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Apr
Résumé
BACKGROUND AND AIMS: Mechanisms regulating M-cell formation are still poorly understood. In vitro studies showed that lymphocytes trigger the conversion of enterocyte cell lines into M cell-like cells on coculture, whereas in vivo their role in M cell differentiation is still elusive. Our aim was first to examine Rag-1-/- mice, lacking B and T lymphocytes, for the presence of intestinal M cells. Second, we investigated the role of lymphotoxin alphabeta signaling on M-cell formation, given its pivotal role in the development of mouse Peyer's patches. METHODS: Small intestines of Rag-1-/- mice, injected or not with soluble lymphotoxin beta receptor-immunoglobulin fusion protein, were analyzed morphologically using whole mount cytochemical staining, immunohistochemistry, and electron microscopy. RESULTS: Small Peyer's patch-like aggregates were found in Rag-1-/- mice in normal number and location. The overlying epithelium of such aggregates was reduced in size but still harbored M cells. In vivo neutralization of lymphotoxin beta-receptor signaling partially reduced the percentage of M cells. CONCLUSIONS: The absence of mature lymphocytes does not prevent the formation of M cells, indicating that the signaling molecules that support M-cell differentiation, such as lymphotoxin alphabeta, may also be supplied by non-B and non-T cells. Mature B lymphocytes, however, are required for the formation of a full-sized follicle-associated epithelium.
Mots-clé
Animals B-Lymphocytes/*immunology Cell Differentiation/immunology Female Homeodomain Proteins/genetics Intestinal Mucosa/*immunology/ultrastructure Intestine, Small/cytology/immunology Lymphotoxin beta Receptor Lymphotoxin-alpha/*immunology Male Mice Mice, Inbred C57BL Mice, Knockout Mice, Nude Microscopy, Electron Microscopy, Electron, Scanning Peyer's Patches/*immunology/ultrastructure Receptors, Tumor Necrosis Factor/immunology Signal Transduction/immunology T-Lymphocytes/*immunology
Pubmed
Web of science
Création de la notice
25/01/2008 16:05
Dernière modification de la notice
20/08/2019 16:07
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