Fragile X mental retardation protein (FMRP) binds specifically to the brain cytoplasmic RNAs BC1/BC200 via a novel RNA-binding motif.

Détails

ID Serval
serval:BIB_A14119855466
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Fragile X mental retardation protein (FMRP) binds specifically to the brain cytoplasmic RNAs BC1/BC200 via a novel RNA-binding motif.
Périodique
The Journal of biological chemistry
Auteur(s)
Zalfa F., Adinolfi S., Napoli I., Kühn-Hölsken E., Urlaub H., Achsel T., Pastore A., Bagni C.
ISSN
0021-9258 (Print)
ISSN-L
0021-9258
Statut éditorial
Publié
Date de publication
30/09/2005
Volume
280
Numéro
39
Pages
33403-33410
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Fragile X mental retardation protein (FMRP), the protein responsible for the fragile X syndrome, is an RNA-binding protein involved in localization and translation of neuronal mRNAs. One of the RNAs known to interact with FMRP is the dendritic non-translatable brain cytoplasmic RNA 1 BC1 RNA that works as an adaptor molecule linking FMRP and some of its regulated mRNAs. Here, we showed that the N terminus of FMRP binds strongly and specifically to BC1 and to its potential human analog BC200. This region does not contain a motif known to specifically recognize RNA and thus constitutes a new RNA-binding motif. We further demonstrated that FMRP recognition involves the 5' stem loop of BC1 and that this is the region that exhibits complementarity to FMRP target mRNAs, raising the possibility that FMRP plays a direct role in BC1/mRNA annealing.

Mots-clé
Base Sequence, Brain/metabolism, Cross-Linking Reagents/pharmacology, Cytoplasm/metabolism, Dendrites/metabolism, Electrophoretic Mobility Shift Assay, Fragile X Syndrome/metabolism, Gene Deletion, Humans, Models, Biological, Molecular Sequence Data, Neurons/metabolism, Protein Structure, Tertiary, RNA, Messenger/genetics, RNA, Messenger/metabolism, RNA, Small Cytoplasmic/chemistry, RNA, Small Cytoplasmic/genetics, RNA, Small Cytoplasmic/metabolism, RNA-Binding Proteins/chemistry, RNA-Binding Proteins/genetics, RNA-Binding Proteins/metabolism, Ultraviolet Rays
Pubmed
Open Access
Oui
Création de la notice
06/03/2017 18:23
Dernière modification de la notice
20/08/2019 16:07
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