Nitric oxide sustains IL-1β expression in human dendritic cells enhancing their capacity to induce IL-17-producing T-cells.

Détails

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Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_A131D9ECEED0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Nitric oxide sustains IL-1β expression in human dendritic cells enhancing their capacity to induce IL-17-producing T-cells.
Périodique
Plos One
Auteur(s)
Obregon C., Graf L., Chung K.F., Cesson V., Nicod L.P.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
10
Numéro
4
Pages
e0120134
Langue
anglais
Résumé
The role played by lung dendritic cells (DCs) which are influenced by external antigens and by their redox state in controlling inflammation is unclear. We studied the role played by nitric oxide (NO) in DC maturation and function. Human DCs were stimulated with a long-acting NO donor, DPTA NONOate, prior to exposure to lipopolysaccharide (LPS). Dose-and time-dependent experiments were performed with DCs with the aim of measuring the release and gene expression of inflammatory cytokines capable of modifying T-cell differentiation, towardsTh1, Th2 and Th17 cells. NO changed the pattern of cytokine release by LPS-matured DCs, dependent on the concentration of NO, as well as on the timing of its addition to the cells during maturation. Addition of NO before LPS-induced maturation strongly inhibited the release of IL-12, while increasing the expression and release of IL-23, IL-1β and IL-6, which are all involved in Th17 polarization. Indeed, DCs treated with NO efficiently induced the release of IL-17 by T-cells through IL-1β. Our work highlights the important role that NO may play in sustaining inflammation during an infection through the preferential differentiation of the Th17 lineage.
Mots-clé
Alkenes/pharmacology, Benzoates/pharmacology, Dendritic Cells/drug effects, Dendritic Cells/immunology, Dose-Response Relationship, Drug, Gene Expression Regulation/drug effects, Humans, Imidazoles/pharmacology, Interleukin-17/biosynthesis, Interleukin-17/secretion, Interleukin-1beta/genetics, Nitric Oxide/pharmacology, T-Lymphocytes/drug effects, T-Lymphocytes/immunology, Time Factors
Pubmed
Web of science
Open Access
Oui
Création de la notice
12/02/2016 15:01
Dernière modification de la notice
20/08/2019 16:07
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