Curcumin inhibits NF-kappaB activation and reduces the severity of experimental steatohepatitis in mice.

Détails

ID Serval
serval:BIB_A0E1018C9300
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Curcumin inhibits NF-kappaB activation and reduces the severity of experimental steatohepatitis in mice.
Périodique
Journal of Hepatology
Auteur(s)
Leclercq I.A., Farrell G.C., Sempoux C., dela Peña A., Horsmans Y.
ISSN
0168-8278 (Print)
ISSN-L
0168-8278
Statut éditorial
Publié
Date de publication
2004
Peer-reviewed
Oui
Volume
41
Numéro
6
Pages
926-934
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
BACKGROUND/AIMS: While oxidative stress is a feature of non-alcoholic steatohepatitis, the causal link between oxidative stress and inflammatory recruitment has yet to be demonstrated. We analysed the role of NF-kappaB redox-sensitive signalling pathway of inflammatory recruitment in experimental steatohepatitis.
METHODS: Mice were fed the methionine and choline deficient (MCD) or the control diet, with or without curcumin, an NF-kappaB inhibitor, for up to 4 weeks. Histopathology, lipoperoxides, NF-kappaB/DNA binding and expression of NF-kappaB-regulated genes were assessed.
RESULTS: MCD-fed mice developed steatohepatitis accompanied by dramatic accumulation of hepatic lipoperoxides, activation of NF-kappaB and induction of pro-inflammatory ICAM-1, COX-2, MCP-1 and CINC mRNA. Curcumin significantly reduced MCD-induced inflammation but had no effect on steatosis or on the level of hepatic lipid peroxides. Curcumin prevented the MCD-induced activation of NF-kappaB and decreased downstream induction of ICAM-1, COX-2 and MCP-1. However, it failed to reduce activation of AP-1, MAPK pathways or CINC expression.
CONCLUSIONS: Curcumin alleviates the severity of hepatic inflammation in experimental steatohepatitis induced by the MCD diet, an effect likely to be mediated via inhibition of NF-kB activation and dependent pro-inflammatory genes. The NF-kappaB pathway is one among several possible signalling pathways by which inflammation is recruited in experimental steatohepatitis.
Mots-clé
Animals, Chemokine CCL2/genetics, Choline Deficiency/complications, Collagen Type I/genetics, Curcumin/pharmacology, Cyclooxygenase 2, Deficiency Diseases/complications, Diet, Enzyme Inhibitors/pharmacology, Fatty Liver/etiology, Fatty Liver/metabolism, Female, Hepatitis/etiology, Hepatitis/metabolism, Inflammation Mediators/metabolism, Intercellular Adhesion Molecule-1/genetics, Methionine/deficiency, Mice, Mice, Inbred C57BL, Mitogen-Activated Protein Kinases/metabolism, NF-kappa B/antagonists & inhibitors, Prostaglandin-Endoperoxide Synthases/genetics, RNA, Messenger/antagonists & inhibitors, Severity of Illness Index, Transcription Factor AP-1/metabolism
Pubmed
Web of science
Création de la notice
20/10/2016 17:22
Dernière modification de la notice
20/08/2019 16:06
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