New model for analysis of mucosal immunity: intestinal secretion of specific monoclonal immunoglobulin A from hybridoma tumors protects against Vibrio cholerae infection

Détails

ID Serval
serval:BIB_A0BEE6A8FE66
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
New model for analysis of mucosal immunity: intestinal secretion of specific monoclonal immunoglobulin A from hybridoma tumors protects against Vibrio cholerae infection
Périodique
Infection and Immunity
Auteur(s)
Winner, L., 3rd , Mack  J., Weltzin  R., Mekalanos  J. J., Kraehenbuhl  J. P., Neutra  M. R.
ISSN
0019-9567 (Print)
Statut éditorial
Publié
Date de publication
03/1991
Volume
59
Numéro
3
Pages
977-82
Notes
Journal Article
Research Support, U.S. Gov't, P.H.S. --- Old month value: Mar
Résumé
Secretory immunoglobulin A (sIgA) plays a role in defense against Vibrio cholerae and other microorganisms that infect mucosal surfaces, but it is not established whether sIgA alone can prevent disease. We report here a strategy for identifying the antigen specificities of monoclonal sIgA antibodies that are capable of providing such protection. IgA hybridomas were generated from Peyer's patch lymphocytes after oral immunization with V. cholerae Ogawa 395. A clone was selected that produced dimeric monoclonal IgA antibodies directed against an Ogawa-specific lipopolysaccharide carbohydrate antigen exposed on the bacterial surface. Hybridoma cells were used to produce subcutaneous "backpack" tumors in syngeneic mice, resulting in secretion of monoclonal sIgA onto mucosal surfaces. Neonatal mice bearing anti-lipopolysaccharide hybridoma backpack tumors were specifically protected against oral challenge with 100 50% lethal doses of virulent Ogawa 395 organisms. Thus, the IgA hybridoma backpack tumor method identifies protective epitopes in the mucosal system and demonstrates that a single monoclonal sIgA can be sufficient to protect against intestinal disease.
Mots-clé
Animals Antibodies, Bacterial/*immunology/secretion Antibodies, Monoclonal/*immunology/secretion Bacterial Adhesion Biological Transport Cholera/immunology/*prevention & control Electrophoresis, Polyacrylamide Gel Epitopes/immunology Hybridomas/*immunology/transplantation Immunity Immunization Immunoglobulin A, Secretory/*immunology/secretion Intestinal Mucosa/*immunology/secretion/ultrastructure Lipopolysaccharides/immunology Mice Mice, Inbred BALB C Models, Biological Peyer's Patches/immunology Vibrio cholerae/immunology/ultrastructure
Pubmed
Web of science
Création de la notice
25/01/2008 15:05
Dernière modification de la notice
20/08/2019 15:06
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