Liquid chromatography/tandem mass spectrometry (highly selective selected reaction monitoring) for the analysis of isopropylthioxanthone in packaged food.

Détails

ID Serval
serval:BIB_9FDA5E4683A4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Liquid chromatography/tandem mass spectrometry (highly selective selected reaction monitoring) for the analysis of isopropylthioxanthone in packaged food.
Périodique
Journal of chromatography. A
Auteur⸱e⸱s
Gallart-Ayala H., Moyano E., Galceran M.T.
ISSN
0021-9673 (Print)
ISSN-L
0021-9673
Statut éditorial
Publié
Date de publication
24/10/2008
Volume
1208
Numéro
1-2
Pages
182-188
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Isopropylthioxanthone (ITX), usually applied as a mixture of 2- and 4-isomers, is a common photo-initiator in UV inks used in paper- or plastic-based packaging materials. In this work a pentafluorophenylpropyl column (HS F5) has been used to achieve the chromatographic separation of the two isomers. A gradient elution with acetonitrile and a 25mM formic acid-ammonium formate at pH 3.75 are required to provide an Rs of 1.3 between the two compounds. The fragmentation pattern of ITX was studied using two mass analyzers, an ion trap (IT) (multi-stage fragmentation) and a triple quadrupole mass analyzer of hyperbolic rods (accurate mass (AM) measurement). The protonated molecule [M+H](+) observed in the mass spectrometry (MS) spectrum lost an isopropyl group, [M+H-C(3)H(6)](+). Later, this ion fragmented, yielding the radical ion [M+H-C(3)H(6)-CHO](+). The elemental composition of these product ions was confirmed by AM measurement. Electrospray ionization (ESI) was used as an ionization source to couple liquid chromatography (LC) to MS. Instrumental quality parameters of three acquisition modes provided by the triple quadrupole mass analyzer were studied and good run-to-run precision (relative standard deviation, RSD, lower than 10%) and limits of detection (LODs) down to 0.8pg injected in the LC-MS/MS system were obtained. Finally the LC-MS/MS method using H-SRM Q1 acquisition mode was used to analyze 2- and 4-ITX in a range of food samples. The use of highly selective selected reaction monitoring (H-SRM on Q1) resulted in improved selectivity without sensitivity loss.

Mots-clé
Chromatography, Liquid/methods, Food Analysis, Food Packaging, Tandem Mass Spectrometry/methods, Thioxanthenes/analysis
Pubmed
Création de la notice
02/06/2017 14:38
Dernière modification de la notice
21/08/2019 6:34
Données d'usage