Clusterin gene expression mediates resistance to apoptotic cell death induced by heat shock and oxidative stress

Détails

ID Serval
serval:BIB_9FD885F15187
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Clusterin gene expression mediates resistance to apoptotic cell death induced by heat shock and oxidative stress
Périodique
Journal of Investigative Dermatology
Auteur⸱e⸱s
Viard  I., Wehrli  P., Jornot  L., Bullani  R., Vechietti  J. L., Schifferli  J. A., Tschopp  J., French  L. E.
ISSN
0022-202X (Print)
Statut éditorial
Publié
Date de publication
03/1999
Volume
112
Numéro
3
Pages
290-6
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar
Résumé
Clusterin is a widely expressed, well conserved, secreted glycoprotein, which is highly induced in tissues regressing as a consequence of apoptotic cell death in vivo. It has recently been shown that clusterin expression is only confined to surviving cells following the induction of apoptosis in vitro, suggesting that it is involved in cell survival rather than death. In the hypothesis that clusterin may be implicated in cellular responses to stress, clusterin gene expression was analyzed in the A431 human epidermoid cancer cell line following heat shock and oxidative stress. Our results show that both a transient heat shock (20 min at 42 degrees C) and various oxidative stresses, including hydrogen peroxide, superoxide anion, hyperoxia and UVA exposure, induce a strong increase in clusterin mRNA levels as assessed by northern blot. Nuclear run-on analysis suggests that transcriptional activation is involved in inducing clusterin mRNA in response to heat shock. Using pulse-chase analysis of control and heat shocked cells, it is shown that clusterin mRNA is translated and secreted, thus resulting in increased extracellular levels of the protein following heat shock. To investigate the function of clusterin in response to these stresses, clusterin anti-sense transfectants that stably express virtually no clusterin at the mRNA and protein level were generated in A431 cells. These anti-sense transfectants are shown to be highly sensitive to apoptotic cell death induced by heat shock or oxidative stress compared with wild-type A431 cells or control transfectants. Taken together, our results show that clusterin gene expression is induced in response to heat shock and oxidative stress in human A431 cells, and confers cellular protection against heat shock and oxidative stress.
Mots-clé
Apoptosis/*physiology Clusterin Gene Expression/*physiology Glycoproteins/*genetics/metabolism *Heat Humans *Molecular Chaperones Oxidative Stress/genetics/*physiology Shock/pathology/*physiopathology Transcription, Genetic/physiology Transfection/physiology Tumor Cells, Cultured
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 16:18
Dernière modification de la notice
20/08/2019 16:06
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