Investigation of a fluorescent reporter microenvironment niche labeling strategy in experimental brain metastasis.
Détails
Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_9FCB37CBD16B
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Institution
Titre
Investigation of a fluorescent reporter microenvironment niche labeling strategy in experimental brain metastasis.
Périodique
iScience
ISSN
2589-0042 (Electronic)
ISSN-L
2589-0042
Statut éditorial
Publié
Date de publication
19/07/2024
Peer-reviewed
Oui
Volume
27
Numéro
7
Pages
110284
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Résumé
Brain metastases are the most common brain tumors in patients and are associated with poor prognosis. Investigating the colonization and outgrowth of brain metastases is challenging given the complexity of the organ, tissue sampling difficulty, and limited experimental models. To address this challenge, we employed a strategy to analyze the metastatic niche in established lesions, based on the release of a cell-penetrating mCherry tag from labeled tumor cells to neighboring niche cells, using different brain metastasis mouse models. We found that CD206+ macrophages were the most abundant cells taking up the mCherry label in established metastases. In vitro and in vivo experiments demonstrated that macrophages uptake and retain the canonical form of mCherry, even without the cell-penetrating portion of the tag. These results identify a specific macrophage subset in the brain that retains tumor-supplied fluorescent molecules, thereby complicating the long-term use of niche labeling strategies in established experimental brain metastasis.
Mots-clé
Cancer, Cell biology, Microenvironment
Pubmed
Open Access
Oui
Création de la notice
25/07/2024 11:00
Dernière modification de la notice
26/07/2024 7:15