Simulations of electrophoretic collisions of DNA knots with gel obstacles

Détails

ID Serval
serval:BIB_9F09EC636701
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Simulations of electrophoretic collisions of DNA knots with gel obstacles
Périodique
Journal of Physics: Condensed Matter
Auteur⸱e⸱s
Weber C., De Los Rios P., Dietler G., Stasiak A.
ISSN
0953-8984
Statut éditorial
Publié
Date de publication
2006
Peer-reviewed
Oui
Volume
18
Numéro
14
Pages
S161-S171
Langue
anglais
Notes
Proceedings Paper
Résumé
Gel electrophoresis can be used to separate nicked circular DNA molecules of equal length but forming different knot types. At low electric fields, complex knots drift faster than simpler knots. However, at high electric field the opposite is the case and simpler knots migrate faster than more complex knots. Using Monte Carlo simulations we investigate the reasons of this reversal of relative order of electrophoretic mobility of DNA molecules forming different knot types. We observe that at high electric fields the simulated knotted molecules tend to hang over the gel fibres and require passing over a substantial energy barrier to slip over the impeding gel fibre. At low electric field the interactions of drifting molecules with the gel fibres are weak and there are no significant energy barriers that oppose the detachment of knotted molecules from transverse gel fibres.
Mots-clé
MOLECULES, MECHANISM, MOBILITY, RECOMBINATION, DIMENSIONS, INVERSION, CATENANES, POLYMERS, MODEL
Web of science
Création de la notice
10/03/2010 9:02
Dernière modification de la notice
20/08/2019 15:05
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