In vitro antigenic modulation of human neuroblastoma cells induced by IFN-gamma, retinoic acid and dibutyryl cyclic AMP

Détails

ID Serval
serval:BIB_9EA5435E61BD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
In vitro antigenic modulation of human neuroblastoma cells induced by IFN-gamma, retinoic acid and dibutyryl cyclic AMP
Périodique
International Journal of Cancer
Auteur⸱e⸱s
Gross  N., Beck  D., Favre  S., Carrel  S.
ISSN
0020-7136
Statut éditorial
Publié
Date de publication
04/1987
Peer-reviewed
Oui
Volume
39
Numéro
4
Pages
521-9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Apr 15
Résumé
Testing with a panel of 26 monoclonal antibodies (MAbs) showed the antigenic profile of 13 human neuroblastoma cell lines to be characterized by a generally poor antigenic expression; therefore, Interferon-gamma (IFN-gamma), dibutyryl cyclic-AMP and retinoic acid were used to analyse the modulation of surface antigenic expression during differentiation. Treatment of neuroblastoma cell lines with IFN-gamma resulted mainly in induction or increase of class-I MHC antigenic expression. Induction of class-II MHC antigens was obtained on only one neuroblastoma cell line out of 13, thus representing an exceptional event. An increase in some other antigens expressed by neuroblastoma cell lines was also observed. In contrast, and in addition to morphological maturation, treatment of these cell lines with the differentiation inducer dibutyryl-cyclic-AMP (dbc-AMP), resulted in general down-modulation of antigenic expression, particularly of neuroblastoma-associated 5A7 or Leu7 antigens. Retinoic acid treatment had no significant effect on MHC antigens, but it decreased expression of 5A7 and Leu7 antigens, and markedly increased the expression of the melanoma-associated antigen Me14-D12. The similarity between the antigenic profile of in vitro differentiated neuroblastoma cells and that of mature ganglioneuroma cells suggests that compounds like cyclic-AMP or retinoic acid are excellent tools for further investigations of the mechanisms of neuroblastoma differentiation and might have important clinical applications.
Mots-clé
Antigens, Neoplasm/*analysis Antigens, Surface/*analysis Bucladesine/*pharmacology Cell Differentiation/drug effects Cell Division/drug effects Cell Line Dose-Response Relationship, Drug HLA Antigens/analysis HLA-DR Antigens/analysis Humans Interferon Type II/*pharmacology Neuroblastoma/*immunology/pathology Phenotype Tretinoin/*pharmacology
Pubmed
Web of science
Création de la notice
20/01/2008 15:55
Dernière modification de la notice
20/08/2019 15:04
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