Isolation and characterization of the rat liver AVP receptor using [125I][d(CH2)5'sarcosine7]AVP.

Détails

ID Serval
serval:BIB_9E01365D13D0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Isolation and characterization of the rat liver AVP receptor using [125I][d(CH2)5'sarcosine7]AVP.
Périodique
Clinical and Experimental Pharmacology and Physiology
Auteur(s)
Trinder D., Kelly J.M., Fernley R., Mooser V., Phillips P.A., Johnston C.I.
ISSN
0305-1870 (Print)
ISSN-L
0305-1870
Statut éditorial
Publié
Date de publication
1992
Volume
19
Numéro
4
Pages
253-260
Langue
anglais
Notes
Publication types: In Vitro ; Journal Article ; Research Support, Non-U.S. Gov't
Résumé
1. A vasopressin (AVP) binding protein was purified from rat liver membranes by an improved method using [125I][d(CH2)5'Sarcosine7]AVP, a selective V1 AVP radioligand and a combination of CHAPS solubilization, gel filtration, lectin affinity and FPLC ion exchange chromatography. 2. The purified protein exhibited a maximum binding activity of 2480 pmol/mg protein with a KD of 4.5 nmol/L, which corresponds to a purification of approximately 26,700-fold. The molecular weight of this protein was 70,000 Da. 3. The binding of [125I][d(CH2)5'Sarcosine7]AVP to the solubilized membranes was dependent on the protein concentration, and was inhibited by the unlabelled peptides [d(CH2)5'Sarcosine7]AVP, AVP, and to a lesser degree by peptides with high V2 receptor affinity, such as 1-desamino-D-AVP and [d(CH2)5'D-Ileu2-Ileu4]AVP. 4. In addition, an AVP anti-idiotypic monoclonal antibody bound to both the partially purified and purified lectin affinity AVP binding protein in a concentration-dependent manner. These results indicate that the purified protein displays similar characteristics to the liver membrane-bound AVP V1 receptor.
Mots-clé
Animals, Antibodies, Monoclonal/diagnostic use, Arginine Vasopressin/analogs & derivatives, Arginine Vasopressin/diagnostic use, Chromatography, High Pressure Liquid, Electrophoresis, Polyacrylamide Gel, Kinetics, Liver/chemistry, Mice, Mice, Inbred BALB C, Molecular Weight, Phospholipids/metabolism, Protein Binding, Rats, Receptors, Angiotensin/drug effects, Receptors, Angiotensin/isolation & purification, Receptors, Vasopressin
Pubmed
Création de la notice
13/10/2013 20:26
Dernière modification de la notice
20/08/2019 15:04
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