Short fluorodeoxyuridine exposure of different human glioblastoma lines induces high-level accumulation of S-phase cells that avidly incorporate 125I-iododeoxyuridine.
Détails
Etat: Public
Version: Final published version
Licence: Non spécifiée
It was possible to publish this article open access thanks to a Swiss National Licence with the publisher.
ID Serval
serval:BIB_9DCEB3C7D983
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Etude de cas (case report): rapporte une observation et la commente brièvement.
Collection
Publications
Institution
Titre
Short fluorodeoxyuridine exposure of different human glioblastoma lines induces high-level accumulation of S-phase cells that avidly incorporate 125I-iododeoxyuridine.
Périodique
European Journal of Nuclear Medicine and Molecular Imaging
ISSN
1619-7070
Statut éditorial
Publié
Date de publication
05/2006
Peer-reviewed
Oui
Volume
33
Numéro
5
Pages
613-620
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article. - Old month value: May
Résumé
PURPOSE: Radio-iododeoxyuridine (IdUrd) is a potential Auger radiation therapy agent incorporated into DNA during the synthesis phase. In this study we sought to optimise S-phase targeting by modulating cellular cycling and radio-IdUrd DNA incorporation using short non-toxic fluorodeoxyuridine (FdUrd) incubations. METHODS: Three human glioblastoma cell lines with different p53 expression were pre-treated with various FdUrd conditions. After different intervals, (125)I-IdUrd DNA incorporation was measured. Fluorescence-activated cell sorter cell cycle analysis was performed after identical intervals post FdUrd pre-treatment. RESULTS: The highest increase in (125)I-IdUrd DNA incorporation was induced by 1-h incubation with 1 muM FdUrd. Increase in radio-IdUrd DNA incorporation was greatest 16-24 h after FdUrd, reaching factors of >or=7.5 over baseline incorporation in the three cell lines. Furthermore, cell synchronisation in S phase was observed with a peak of >or=69.5% in the three cell lines at 16 and 24 h post FdUrd, corresponding to an increase of 2.5-4.1 over baseline. CONCLUSION: FdUrd-induced thymidine synthesis inhibition led to S-phase accumulation that was maximal after an interval of 16-24 h and time-correlated with the highest radio-IdUrd DNA incorporation. These observations might allow the rational design of an Auger radiation therapy targeting a maximal number of S-phase cells in single treatment cycles.
Mots-clé
Cell Cycle/drug effects, Cell Cycle/radiation effects, Cell Line, Tumor, Dose-Response Relationship, Drug, Floxuridine/administration & dosage, Glioblastoma/metabolism, Glioblastoma/pathology, Humans, Idoxuridine/pharmacokinetics, Iodine Radioisotopes/pharmacokinetics, Metabolic Clearance Rate/drug effects, Radiopharmaceuticals/pharmacokinetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 12:28
Dernière modification de la notice
14/02/2022 8:56