Inhibition of T cell-mediated cytolysis by 2-deoxy-D-glucose:dissociation of the inhibitory effect from glycoprotein synthesis
Détails
ID Serval
serval:BIB_9DA992A55F37
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Inhibition of T cell-mediated cytolysis by 2-deoxy-D-glucose:dissociation of the inhibitory effect from glycoprotein synthesis
Périodique
European Journal of Immunology
ISSN
0014-2980 (Print)
Statut éditorial
Publié
Date de publication
06/1979
Volume
9
Numéro
6
Pages
466-70
Notes
Journal Article --- Old month value: Jun
Résumé
Previous studies have established that T cell-mediated cytolysis can be reversibly inhibited by the hexose analogue 2-deoxy-D-glucose (2-DG) by a mechanism which is apparently unrelated to energy depletion. The possibility that the inhibitory effect of 2-DG on cytolysis was linked to its known inhibitory effect on glycoprotein synthesis was therefore investigated. In contrast to the results obtained with 2-DG, no inhibition of cytolysis was observed in the presence of tunicamycin, a potent and specific inhibitor of lipid carrier-dependent protein glycosylation. Furthermore, populations of cytolytic cells which had been pretreated with doses of tunicamycin sufficient to block the incorporation of mannose (or 2-DG) into glycoproteins were still fully susceptible to inhibition by 2-DG. Other known inhibitors of viral protein glycosylation, such as glucosamine and galactosamine, inhibited cytolysis only weakly under conditions where 2-DG was highly effective. Kinetic studies revealed that the inhibitory effect of 2-DG on cytolysis could be reversed within minutes by the addition of exogenous glucose. Furthermore, suggestive evidence was obtained that inhibition cytolysis by 2-DG was linked to a parallel inhibition of effector: target cell binding. Taken together, these results strongly suggest that the inhibitory effect of 2-DG on cytolysis can be dissociated from its effect on protein glycosylation. An alternative mechanism of action of 2-DG is suggested.
Mots-clé
Animals
Cytotoxicity, Immunologic/*drug effects
Deoxy Sugars/*pharmacology
Deoxyglucose/*pharmacology
Galactosamine/pharmacology
Glucosamine/pharmacology
Glycoproteins/*biosynthesis
Mice
Mice, Inbred C57BL
Mice, Inbred DBA
T-Lymphocytes/*immunology
Time Factors
Tunicamycin/pharmacology
Pubmed
Web of science
Création de la notice
28/01/2008 12:14
Dernière modification de la notice
20/08/2019 16:04