Longitudinal investigation of the metabolome of 3D aggregating brain cell cultures at different maturation stages by <sup>1</sup>H HR-MAS NMR.

Détails

ID Serval
serval:BIB_9CD73DA5A938
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Longitudinal investigation of the metabolome of 3D aggregating brain cell cultures at different maturation stages by <sup>1</sup>H HR-MAS NMR.
Périodique
Analytical and bioanalytical chemistry
Auteur⸱e⸱s
Diserens G., Vermathen M., Zurich M.G. (co-dernier), Vermathen P.
ISSN
1618-2650 (Electronic)
ISSN-L
1618-2642
Statut éditorial
Publié
Date de publication
10/2018
Peer-reviewed
Oui
Volume
410
Numéro
26
Pages
6733-6749
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
The aim of the present study was to establish the developmental profile of metabolic changes of 3D aggregating brain cell cultures by <sup>1</sup> H high-resolution magic angle spinning (HR-MAS) NMR spectroscopy. The histotypic 3D brain aggregate, containing all brain cell types, is an excellent model for mechanistic studies including OMICS analysis; however, their metabolic profile has not been yet fully investigated. Chemometric analysis revealed a clear separation of samples from the different maturation time points. Metabolite concentration evolutions could be followed and revealed strong and various metabolic alterations. The strong metabolite evolution emphasizes the brain modeling complexity during maturation, possibly reflecting physiological processes of brain tissue development. The small observed intra- and inter-experimental variabilities show the robustness of the combination of <sup>1</sup> H-HR-MAS NMR and 3D brain aggregates, making it useful to investigate mechanisms of toxicity that will ultimately contribute to improve predictive neurotoxicology. Graphical Abstract ᅟ.
Mots-clé
Analytical Chemistry, Biochemistry, 3D brain cell cultures, Brain aggregates, Chemometrics, High-resolution magic angle spinning, Nuclear magnetic resonance
Pubmed
Web of science
Création de la notice
13/08/2018 8:31
Dernière modification de la notice
17/01/2020 6:18
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