Are the light-harvesting I complexes from Rhodospirillum rubrum arranged around the reaction centre in a square geometry?

Détails

ID Serval
serval:BIB_9CA9E808D262
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Are the light-harvesting I complexes from Rhodospirillum rubrum arranged around the reaction centre in a square geometry?
Périodique
Journal of molecular biology
Auteur⸱e⸱s
Stahlberg H., Dubochet J., Vogel H., Ghosh R.
ISSN
0022-2836
Statut éditorial
Publié
Date de publication
1998
Peer-reviewed
Oui
Volume
282
Numéro
4
Pages
819-31
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Résumé
The basic photosynthetic unit containing the reaction centre and the light-harvesting I complex (RC-LHI) of the purple non-sulphur bacterium Rhodospirillum rubrum was purified and reconstituted into two-dimensional (2D) membrane crystals. Transmission electron microscopy using conventional techniques and cryoelectron microscopy of the purified single particles and of 2D crystals yielded a projection of the RC-LHI complex at a resolution of at least 1.6 nm. In this projection the LHI ring appears to have a square symmetry and packs in a square crystal lattice. The square geometry of the LHI ring was observed also in images of single isolated particles of the RC-LHI complex. However, although the LHI units are packed identically within the crystal lattice, a new rotational analysis developed here showed that the reaction centres take up one of four possible orientations within the ring. This fourfold disorder supports our interpretation of a square ring symmetry and suggests that a hitherto undetected component may be present within the photosynthetic unit.
Mots-clé
Bacterial Chromatophores, Bacterial Proteins, Cryoelectron Microscopy, Crystallization, Hydrogen-Ion Concentration, Light-Harvesting Protein Complexes, Microdialysis, Microscopy, Electron, Molecular Weight, Phospholipid Ethers, Photosynthetic Reaction Center Complex Proteins, Protein Conformation, Rhodospirillum rubrum, Solubility, Spectrum Analysis
Pubmed
Web of science
Création de la notice
24/01/2008 11:25
Dernière modification de la notice
20/08/2019 16:03
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