Functional analyses of a N-terminal splice variant of the alpha subunit of the epithelial sodium channel

Détails

ID Serval
serval:BIB_9C9D14B997FE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Functional analyses of a N-terminal splice variant of the alpha subunit of the epithelial sodium channel
Périodique
Cellular Physiology and Biochemistry
Auteur(s)
Chraibi A., Verdumo C., Mérillat A. M., Rossier B. C., Horisberger J. D., Hummler E.
ISSN
1015-8987 (Print)
Statut éditorial
Publié
Date de publication
2001
Volume
11
Numéro
3
Pages
115-22
Notes
Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.
Résumé
The amiloride-sensitive epithelial sodium channel (ENaC) is the limiting step for sodium absorption in epithelial cells of the distal nephron, distal colon, airways and excretory ducts of several glands. In vivo and in vitro studies showed that the alpha subunit of ENaC is necessary for the expression of functional channels. Using RT-PCR strategy, a novel N-terminal splice variant has been identified which deletes 49 amino acids in the N-terminal region of the mouse alphaENaC subunit. In oocytes expressing the alphaENaC splice variant, together with beta and gammaENaC subunits, amiloride-sensitive currents were less than 20% of values obtained with the wild type ENaC. The single channel conductance and the ionic selectivity were similar and there was only a minor decrease in the level of expression of the protein at the oocyte surface. These findings indicate that the deleted sequence in the N-terminal part of the mouse and rat alphaENaC subunit might play a role in the regulation of the activity of expressed ENaC channels.
Mots-clé
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA Primers Epithelial Sodium Channel Membrane Potentials Mice Mice, Inbred C57BL Molecular Sequence Data Mutagenesis, Site-Directed Patch-Clamp Techniques *RNA Splicing Sequence Homology, Amino Acid Sodium Channels/chemistry/genetics/*physiology
Pubmed
Web of science
Création de la notice
24/01/2008 13:38
Dernière modification de la notice
20/08/2019 16:03
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