The melanocytic protein Melan-A/MART-1 has a subcellular localization distinct from typical melanosomal proteins
Détails
ID Serval
serval:BIB_9C1D5A6D7A4D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
The melanocytic protein Melan-A/MART-1 has a subcellular localization distinct from typical melanosomal proteins
Périodique
Traffic
ISSN
1398-9219 (Print)
Statut éditorial
Publié
Date de publication
09/2002
Volume
3
Numéro
9
Pages
678-93
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep
Research Support, Non-U.S. Gov't --- Old month value: Sep
Résumé
To delineate the role of the melanocyte lineage-specific protein Melan-A/MART-1 in melanogenic functions, a set of biochemical and microscopical studies was performed. Biochemical analysis revealed that Melan-A/MART-1 is post-translationally acylated and undergoes a rapid turnover in a pigmented melanoma cell line. Immunofluorescence and immunoelectron microscopy analyses indicated that Melan-A/MART-1 is mainly located in the Golgi area and only partially colocalizes with melanosomal proteins. Quantitative immunoelectron microscopy showed that the highest proportion of the cellular content of Melan-A/MART-1 was found in small vesicles and tubules throughout the cell, whereas the concentration was maximal in the Golgi region, particularly the trans-Golgi network. Substantial labeling was also present on melanosomes, endosomes, ER, nuclear envelope, and plasma membrane. In early endosomes, Melan-A was enriched in areas of the limiting membrane covered by a bi-layered coat, a structural characteristic of melanosomal precursor compartments. Upon melanosome maturation, Melan-A concentration decreased and its predominant localization shifted from the limiting membrane to internal vesicle membranes. In conjunction with its acylation, the high expression levels of Melan-A in the trans-Golgi network, in dispersed vesicles, and on the limiting membrane of premelanosomes indicate that the protein may play a role during the early stage of melanosome biogenesis.
Mots-clé
Antigens, Neoplasm
Electrophoresis, Polyacrylamide Gel
Golgi Apparatus/metabolism
Humans
Melanosomes/*metabolism
Microscopy, Confocal
Microscopy, Fluorescence
Neoplasm Proteins/*metabolism
Skin/metabolism
Subcellular Fractions/*metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 12:17
Dernière modification de la notice
20/08/2019 16:02