Actin filaments accumulated in the nucleus remain in the vicinity of condensing chromosomes in the zebrafish early embryo.
Détails
Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_9BD9D3EE2E70
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Actin filaments accumulated in the nucleus remain in the vicinity of condensing chromosomes in the zebrafish early embryo.
Périodique
Biology open
ISSN
2046-6390 (Electronic)
ISSN-L
2046-6390
Statut éditorial
Publié
Date de publication
15/05/2023
Peer-reviewed
Oui
Volume
12
Numéro
5
Pages
bio059783
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
In the cytoplasm, filamentous actin (F-actin) plays a critical role in cell regulation, including cell migration, stress fiber formation, and cytokinesis. Recent studies have shown that actin filaments that form in the nucleus are associated with diverse functions. Here, using live imaging of an F-actin-specific probe, superfolder GFP-tagged utrophin (UtrCH-sfGFP), we demonstrated the dynamics of nuclear actin in zebrafish (Danio rerio) embryos. In early zebrafish embryos up to around the high stage, UtrCH-sfGFP increasingly accumulated in nuclei during the interphase and reached a peak during the prophase. After nuclear envelope breakdown (NEBD), patches of UtrCH-sfGFP remained in the vicinity of condensing chromosomes during the prometaphase to metaphase. When zygotic transcription was inhibited by injecting α-amanitin, the nuclear accumulation of UtrCH-sfGFP was still observed at the sphere and dome stages, suggesting that zygotic transcription may induce a decrease in nuclear F-actin. The accumulation of F-actin in nuclei may contribute to proper mitotic progression of large cells with rapid cell cycles in zebrafish early embryos, by assisting in NEBD, chromosome congression, and/or spindle assembly.
Mots-clé
Animals, Zebrafish, Actins, Chromosomes/genetics, Mitosis, Actin Cytoskeleton, Live-cell imaging, Nuclear actin, Zebrafish embryo
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/04/2023 14:50
Dernière modification de la notice
08/08/2024 7:37