Analysis of hemoglobin-based oxygen carriers by CE-UV/Vis and CE-ESI-TOF/MS.

Détails

ID Serval
serval:BIB_9BC236E2CF10
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Analysis of hemoglobin-based oxygen carriers by CE-UV/Vis and CE-ESI-TOF/MS.
Périodique
Electrophoresis
Auteur⸱e⸱s
Staub A., Rudaz S., Saugy M., Veuthey J.L., Schappler J.
ISSN
1522-2683[electronic], 0173-0835[linking]
Statut éditorial
Publié
Date de publication
2010
Peer-reviewed
Oui
Volume
31
Numéro
7
Pages
1241-1247
Langue
anglais
Résumé
Blood doping involves the use of products that enhance the uptake, transport, or delivery of oxygen to the blood. One approach uses artificial oxygen carriers, known as hemoglobin-based oxygen carriers (HBOCs). This study describes an analytical strategy based on CE for detecting intact HBOCs in plasma samples collected for doping control. On-capillary detection was performed by UV/Vis at 415 nm, which offered detection selectivity for hemoproteins (such as hemoglobin and HBOCs). On-line ESI-MS detection with a TOF analyzer was further used to provide accurate masses on CE peaks and to confirm the presence of HBOCs. An immunodepletion sample preparation step was mandatory prior to analysis, in order to remove most abundant proteins that interfered with CE separation and altered the ESI process. This analytical method was successfully applied to plasma samples enriched with Oxyglobin, a commercially available HBOC used for veterinary purposes. Detection limits of 0.20 and 0.45 g/dL were achieved in plasma for CE-UV/Vis at 415 nm and CE-ESI-TOF/MS, respectively.
Mots-clé
Blood Substitutes/chemistry, Doping in Sports, Electrophoresis, Capillary/methods, Hemoglobins/chemistry, Humans, Immunosorbent Techniques, Proteins/isolation & purification, Sensitivity and Specificity, Spectrometry, Mass, Electrospray Ionization/methods, Spectrophotometry, Ultraviolet
Pubmed
Web of science
Création de la notice
11/03/2011 16:26
Dernière modification de la notice
20/08/2019 15:02
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