Creation of nanostructures to study the topographical dependency of protein adsorption

Détails

ID Serval
serval:BIB_9B9FBAE8F797
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Creation of nanostructures to study the topographical dependency of protein adsorption
Périodique
Colloids and Surfaces B: Biointerfaces
Auteur(s)
Galli C., Collaud Coen M., Hauert R., Katanaev V.L., Gröning P., Schlapbach L.
ISSN
0927-7765 (Print)
Statut éditorial
Publié
Date de publication
2002
Peer-reviewed
Oui
Volume
26
Numéro
3
Pages
255-267
Langue
anglais
Résumé
Nanostructures of sizes comparable to protein dimensions are created on Si and Ti surfaces by local anodic oxidation (LAO) using the atomic force microscope (AFM). The characterization of the surface by X-ray photoelectron spectroscopy (XPS) reveals that this method assures a modification of the topography of the surface without a change of its chemical composition. Surfaces structured by LAO therefore represent ideal systems to study the dependence of protein adsorption on topography. We are able to visualize the created nanostructures with an AFM and successively adsorb the proteins in situ, rinse and image the new surface. The densities of adsorbed proteins on the nanostructured and neat surfaces are compared and we find that the protein arrangement depends on the underlying nanostructures, showing that proteins can "sense" the topography of surfaces at the nanometer scale. This result can be considered as the nanoscale analogous of the adsorption found for cell systems on micrometer structures. (C) 2002 Elsevier Science B.V. All rights reserved.
Mots-clé
nanostructures, topography, protein, adsorption, surface
Web of science
Création de la notice
09/07/2012 10:08
Dernière modification de la notice
20/08/2019 16:02
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