Neofunctionalization in vertebrates: the example of retinoic acid receptors.

Détails

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Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_9B994D87AC3D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Neofunctionalization in vertebrates: the example of retinoic acid receptors.
Périodique
PLoS Genetics
Auteur(s)
Escriva H., Bertrand S., Germain P., Robinson-Rechavi M., Umbhauer M., Cartry J., Duffraisse M., Holland L., Gronemeyer H., Laudet V.
ISSN
1553-7404 (Electronic)
ISSN-L
1553-7390
Statut éditorial
Publié
Date de publication
2006
Peer-reviewed
Oui
Volume
2
Numéro
7
Pages
e102
Langue
anglais
Résumé
Understanding the role of gene duplications in establishing vertebrate innovations is one of the main challenges of Evo-Devo (evolution of development) studies. Data on evolutionary changes in gene expression (i.e., evolution of transcription factor-cis-regulatory elements relationships) tell only part of the story; protein function, best studied by biochemical and functional assays, can also change. In this study, we have investigated how gene duplication has affected both the expression and the ligand-binding specificity of retinoic acid receptors (RARs), which play a major role in chordate embryonic development. Mammals have three paralogous RAR genes--RAR alpha, beta, and gamma--which resulted from genome duplications at the origin of vertebrates. By using pharmacological ligands selective for specific paralogues, we have studied the ligand-binding capacities of RARs from diverse chordates species. We have found that RAR beta-like binding selectivity is a synapomorphy of all chordate RARs, including a reconstructed synthetic RAR representing the receptor present in the ancestor of chordates. Moreover, comparison of expression patterns of the cephalochordate amphioxus and the vertebrates suggests that, of all the RARs, RAR beta expression has remained most similar to that of the ancestral RAR. On the basis of these results together, we suggest that while RAR beta kept the ancestral RAR role, RAR alpha and RAR gamma diverged both in ligand-binding capacity and in expression patterns. We thus suggest that neofunctionalization occurred at both the expression and the functional levels to shape RAR roles during development in vertebrates.
Mots-clé
Amino Acid Sequence, Animals, Evolution, Molecular, Gene Expression Regulation, Developmental, Lampreys, Ligands, Molecular Sequence Data, Mutation, Phylogeny, Receptors, Retinoic Acid/metabolism, Retinoids/metabolism, Sequence Homology, Amino Acid, Transcription, Genetic, Transcriptional Activation, Xenopus
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 17:46
Dernière modification de la notice
20/08/2019 15:02
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