Assessment of nuclear size, nuclear DNA content and proliferation index in stereotaxic biopsies from brain tumours.

Détails

ID Serval
serval:BIB_9A858151E0ED
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Assessment of nuclear size, nuclear DNA content and proliferation index in stereotaxic biopsies from brain tumours.
Périodique
Neuropathology and Applied Neurobiology
Auteur(s)
Salmon I., Levivier M., Camby I., Rombaut K., Gras T., Pasteels J.L., Brotchi J., Kiss R.
ISSN
0305-1846 (Print)
ISSN-L
0305-1846
Statut éditorial
Publié
Date de publication
1993
Peer-reviewed
Oui
Volume
19
Numéro
6
Pages
507-518
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
In this paper we study the feasibility of measuring the diverse biological parameters in stereotaxic biopsies from human brain lesions. These biological parameters are the nuclear area (NA), the proliferation index (PI) and the ploidy level, the latter of which was evaluated by means of the DNA index (DI) and histogram type (DHT). These parameters were assessed by means of the digital cell image analysis of Feulgen-stained nuclei. This analysis was performed on 124 samples from 22 computed tomography (CT)-guided stereotaxic biopsies. The data show that the methodology used here enables the above parameters to be assessed on small samples without limiting the classical anatomopathological diagnosis. The data also reveal that the DHT corresponded more accurately to the ploidy level in the sample analysed than the DI. Lastly, it appears that supratentorial astrocytic tumours of the adult, which constituted the majority of the cases analysed here, are strongly heterogeneous at a biological level.
Mots-clé
Biopsy/methods, Brain/pathology, Brain Neoplasms/genetics, Brain Neoplasms/pathology, Cell Division, Cell Nucleus/metabolism, Cell Nucleus/ultrastructure, DNA, Neoplasm/metabolism, Feasibility Studies, Humans, Image Processing, Computer-Assisted, Ploidies, Stereotaxic Techniques
Pubmed
Web of science
Création de la notice
20/01/2008 18:35
Dernière modification de la notice
20/08/2019 16:01
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