Staphylococcal enterotoxin-dependent lysis of MHC class II negative target cells by cytolytic T lymphocytes

Détails

ID Serval
serval:BIB_9A6855F2A76E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Staphylococcal enterotoxin-dependent lysis of MHC class II negative target cells by cytolytic T lymphocytes
Périodique
Journal of Immunology
Auteur(s)
Herrmann  T., Romero  P., Sartoris  S., Paiola  F., Accolla  R. S., Maryanski  J. L., MacDonald  H. R.
ISSN
0022-1767 (Print)
Statut éditorial
Publié
Date de publication
04/1991
Volume
146
Numéro
8
Pages
2504-12
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Apr 15
Résumé
The enterotoxins of Staphylococcus aureus (SE) are extremely potent activators of human and mouse T lymphocytes. In general, T cell responses to SE are MHC class II dependent (presumably reflecting the ability of SE to bind directly to MHC class II molecules) and restricted to responding cells expressing certain T cell receptor beta-chain variable (TCR V beta) domains. Recently we demonstrated that CD8+ CTL expressing appropriate TCR V beta could recognize SE presented on MHC class II-bearing target cells. We now show that MHC class II expression is not strictly required for T cell recognition of SE. Both human and mouse MHC class II negative target cells could be recognized (i.e., lysed) in a SE-dependent fashion by CD8+ mouse CTL clones and polyclonal populations, provided that the CTL expressed appropriate TCR V beta elements. SE-dependent lysis of MHC class II negative targets by CTL was inhibited by mAb directed against CD3 or LFA-1, suggesting that SE recognition was TCR and cell contact dependent. Furthermore, different SE were recognized preferentially by CTL on MHC class II+ vs MHC class II- targets. Taken together, our data raise the possibility that SE binding structures distinct from MHC class II molecules may exist.
Mots-clé
Animals Blotting, Northern Cell Line *Cytotoxicity, Immunologic Dose-Response Relationship, Immunologic Enterotoxins/*immunology Epitopes Flow Cytometry Histocompatibility Antigens Class II/*immunology Humans Lymphocyte Activation/drug effects Lymphoma, B-Cell/immunology Mast-Cell Sarcoma/immunology Mice Mice, Inbred BALB C *Staphylococcus aureus T-Lymphocytes, Cytotoxic/*immunology
Pubmed
Web of science
Création de la notice
28/01/2008 12:28
Dernière modification de la notice
20/08/2019 16:01
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