Isolation of prolactin-producing cells from first and second trimester decidua

Détails

ID Serval
serval:BIB_9A26D030B7A9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Isolation of prolactin-producing cells from first and second trimester decidua
Périodique
Journal of Clinical Endocrinology and Metabolism
Auteur⸱e⸱s
Braverman  M. B., Bagni  A., de Ziegler  D., Den  T., Gurpide  E.
ISSN
0021-972X
Statut éditorial
Publié
Date de publication
03/1984
Peer-reviewed
Oui
Volume
58
Numéro
3
Pages
521-5
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Mar
Résumé
Two cell types isolated and purified to homogeneity from human decidua obtained at 8-17 weeks of gestation were shown immunocytochemically to correspond to decidual and epithelial cells in the tissue of origin. The decidual cells reacted with antihuman PRL antiserum, and epithelial cells reacted with antiserum against keratin, an epithelial cell marker. Decidual and epithelial cells were cultured separately to determine their abilities to release PRL to the medium. Decidual cells released 140-410 ng PRL/mg protein in 24 h, whereas no PRL was detectable in cultures of isolated epithelial cells. These homogeneous preparations provide an excellent system with which to study the regulation of PRL production and other biochemical properties of decidual components.
Mots-clé
Adult Cell Separation/methods Decidua/cytology/*metabolism/ultrastructure Epithelium/metabolism Female Histocytochemistry Humans Immunoenzyme Techniques Pregnancy Pregnancy Trimester, First Pregnancy Trimester, Third Prolactin/*biosynthesis Radioimmunoassay
Pubmed
Web of science
Création de la notice
28/02/2008 12:37
Dernière modification de la notice
20/08/2019 16:01
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