Naturally occurring human lymphocyte antigen-A2 restricted CD8+ T-cell response to the cancer testis antigen NY-ESO-1 in melanoma patients.
Détails
ID Serval
serval:BIB_99DF5B4C6096
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Naturally occurring human lymphocyte antigen-A2 restricted CD8+ T-cell response to the cancer testis antigen NY-ESO-1 in melanoma patients.
Périodique
Cancer research
ISSN
0008-5472
Statut éditorial
Publié
Date de publication
08/2000
Peer-reviewed
Oui
Volume
60
Numéro
16
Pages
4499-4506
Langue
anglais
Notes
Publication types: Journal Article ; Research Support
Résumé
Cancer testis (CT) antigens are particularly interesting candidates for cancer vaccines. However, T-cell reactivity to CT antigens has been detected only occasionally in cancer patients, even after vaccination. A new group of CT antigens has been recently identified using the SEREX technique based on immunoscreening of tumor cDNA expression libraries with autologous sera. We have used fluorescent HLA-A2/peptide tetramers containing an optimized antigenic peptide to directly identify HLA-A2-restricted CD8+ T cells specific for the SEREX-defined CT antigen NY-ESO-1 in melanoma patients. High frequencies of NY-ESO-1-specific CD8+ T cells were readily detected in peptide-stimulated peripheral blood mononuclear cells as well as in lymphocytes infiltrating melanoma lesions from patients with measurable antibody responses to NY-ESO-1. NY-ESO-1-specific CD8+ T cells were also detectable in peptide-stimulated peripheral blood mononuclear cells from some seronegative patients. Whereas the frequencies of NY-ESO-1-specific CD8+ T cells in circulating lymphocytes were usually below the limit of detection by tetramer staining, the presence of NY-ESO-1 CD8+ T cells displaying a memory phenotype was clearly detectable ex vivo in blood from a seropositive patient over an extended period of time. These results indicate that sustained CD8+ T-cell responses to CT antigens can naturally occur both locally and systemically in melanoma patients.
Mots-clé
Amino Acid Sequence, Antigens, Neoplasm/immunology, CD8-Positive T-Lymphocytes/immunology, Cancer Vaccines/immunology, Epitopes, T-Lymphocyte/immunology, HLA-A2 Antigen/immunology, Humans, Lymphocyte Activation/immunology, Lymphocytes, Tumor-Infiltrating/immunology, Male, Melanoma/blood, Melanoma/immunology, Membrane Proteins, Peptide Fragments/immunology, Proteins/immunology, T-Lymphocytes, Cytotoxic/immunology, Testis/immunology
Pubmed
Web of science
Création de la notice
28/01/2008 12:14
Dernière modification de la notice
20/08/2019 16:01