Human immunodeficiency virus type 1 causes productive infection of macrophages in primary placental cell cultures.

Détails

ID Serval
serval:BIB_99A8ACE7FA60
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Human immunodeficiency virus type 1 causes productive infection of macrophages in primary placental cell cultures.
Périodique
Journal of Infectious Diseases
Auteur⸱e⸱s
McGann K.A., Collman R., Kolson D.L., Gonzalez-Scarano F., Coukos G., Coutifaris C., Strauss J.F., Nathanson N.
ISSN
0022-1899 (Print)
ISSN-L
0022-1899
Statut éditorial
Publié
Date de publication
1994
Volume
169
Numéro
4
Pages
746-753
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.Publication Status: ppublish
Résumé
To characterize the role of the placenta in vertical transmission of human immunodeficiency virus type 1 (HIV-1), the susceptibility of primary human placental cultures and of transformed trophoblast cell lines to infection by several HIV-1 isolates was examined. Placental cultures supported the replication of all strains tested, including lymphocyte-, macrophage-, and amphotropic isolates. All viruses replicated to modest levels, with production of both viral antigen and infectious virus in the culture supernatants. Placental cells demonstrated a pattern of permissiveness for HIV-1 isolates distinct from that seen with lymphocytes, blood-derived macrophages, or T cell lines. Immunofluorescent staining showed that 5%-10% of the cultured placental cells expressed viral antigens, and double labeling revealed that the HIV-positive cells were macrophages not trophoblasts. None of the trophoblast cell line (JEG-3, Jar, BeWo, HP-W1) could be infected by HIV. These results support the hypothesis that infection of the placenta could play a role in maternofetal transmission of HIV-1 and suggest that the placental macrophage is likely to be the primary cell type responsible.
Mots-clé
Cell Line, Transformed, Cells, Cultured, Choriocarcinoma, Female, Fluorescent Antibody Technique, HIV Antigens/biosynthesis, HIV-1/physiology, HeLa Cells, Humans, Macrophages/microbiology, Placenta/cytology, Placenta/microbiology, Pregnancy, Trophoblasts/microbiology, Tumor Cells, Cultured, Virus Replication
Pubmed
Web of science
Création de la notice
14/10/2014 11:43
Dernière modification de la notice
20/08/2019 15:01
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