Development of a high throughput PCR to detect Coxiella burnetii and its application in a diagnostic laboratory over a 7-year period.

Détails

Ressource 1Télécharger: BIB_997C3FE84771.P001.pdf (458.15 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_997C3FE84771
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Development of a high throughput PCR to detect Coxiella burnetii and its application in a diagnostic laboratory over a 7-year period.
Périodique
New Microbes and New Infections
Auteur(s)
Jaton K., Peter O., Raoult D., Tissot J.D., Greub G.
ISSN
2052-2975 (Electronic)
ISSN-L
2052-2975
Statut éditorial
Publié
Date de publication
2013
Peer-reviewed
Oui
Volume
1
Numéro
1
Pages
6-12
Langue
anglais
Résumé
Q fever is a worldwide zoonotic infectious disease due to Coxiella burnetii. The clinical presentation may be acute (pneumonia and/or hepatitis) or chronic (most commonly endocarditis). Diagnosis mainly relies on serology and PCR. We therefore developed a quantitative real-time PCR. We first tested blindly its performance on various clinical samples and then, when thoroughly validated, we applied it during a 7-year period for the diagnosis of both acute and persistent C. burnetii infection. Analytical sensitivity (< 10 copies/PCR) was excellent. When tested blindly on 183 samples, the specificity of the PCR was 100% (142/142) and the sensitivity was 71% (29/41). The sensitivity was 88% (7/8) on valvular samples, 69% (20/29) on blood samples and 50% (2/4) on urine samples. This new quantitative PCR was then successfully applied for the diagnosis of acute Q fever and endovascular infection due to C. burnetii, allowing the diagnosis of Q fever in six patients over a 7-year period. During a local small cluster of cases, the PCR was also applied to blood from 1355 blood donors; all were negative confirming the high specificity of this test. In conclusion, we developed a highly specific method with excellent sensitivity, which may be used on sera for the diagnosis of acute Q fever and on various samples such as sera, valvular samples, aortic specimens, bone and liver, for the diagnosis of persistent C. burnetii infection.
Mots-clé
Blood donations, Coxiella burnetii , high throughput, PCR , Q fever
Pubmed
Open Access
Oui
Création de la notice
11/08/2013 16:03
Dernière modification de la notice
20/08/2019 15:00
Données d'usage