Defining signatures of peripheral T-cell lymphoma with a targeted 20-marker gene expression profiling assay.

Détails

Ressource 1Télécharger: 31488561.pdf (2985.83 [Ko])
Etat: Public
Version: Author's accepted manuscript
Licence: Non spécifiée
Document(s) secondaire(s)
Télécharger: 1582.full.pdf (3337.98 [Ko])
Etat: Public
Version: Final published version
Licence: Non spécifiée
ID Serval
serval:BIB_99596E330272
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Defining signatures of peripheral T-cell lymphoma with a targeted 20-marker gene expression profiling assay.
Périodique
Haematologica
Auteur⸱e⸱s
Drieux F. (co-premier), Ruminy P. (co-premier), Abdel-Sater A., Lemonnier F., Viailly P.J., Fataccioli V., Marchand V., Bisig B., Letourneau A., Parrens M., Bossard C., Bruneau J., Dobay P., Veresezan L., Dupuy A., Pujals A., Robe C., Sako N., Copie-Bergman C., Delfau-Larue M.H., Picquenot J.M., Tilly H., Delarue R., Jardin F. (co-dernier), de Leval L. (co-dernier), Gaulard P. (co-dernier), Gaulard P. (co-dernier)
ISSN
1592-8721 (Electronic)
ISSN-L
0390-6078
Statut éditorial
Publié
Date de publication
06/2020
Peer-reviewed
Oui
Volume
105
Numéro
6
Pages
1582-1592
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Peripheral T-cell lymphoma comprises a heterogeneous group of mature non-Hodgkin lymphomas. Their diagnosis is challenging, with up to 30% of cases remaining unclassifiable and referred to as "not otherwise specified". We developed a reverse transcriptase-multiplex ligation-dependent probe amplification gene expression profiling assay to differentiate the main T-cell lymphoma entities and to study the heterogeneity of the "not specified" category. The test evaluates the expression of 20 genes, including 17 markers relevant to T-cell immunology and lymphoma biopathology, one Epstein-Barr virus-related transcript, and variants of RHOA (G17V) and IDH2 (R172K/T). By unsupervised hierarchical clustering, our assay accurately identified 21 of 21 ALK-positive anaplastic large cell lymphomas, 16 of 16 extranodal natural killer (NK)/T-cell lymphomas, 6 of 6 hepatosplenic T-cell lymphomas, and 13 of 13 adult T-cell leukemia/lymphomas. ALK-negative anaplastic lymphomas (n=34) segregated into one cytotoxic cluster (n=10) and one non-cytotoxic cluster expressing Th2 markers (n=24) and enriched in DUSP22-rearranged cases. The 63 T <sub>FH</sub> -derived lymphomas divided into two subgroups according to a predominant T <sub>FH</sub> (n=50) or an enrichment in Th2 (n=13) signatures. We next developed a support vector machine predictor which attributed a molecular class to 27 of 77 not specified T-cell lymphomas: 17 T <sub>FH</sub> , five cytotoxic ALK-negative anaplastic and five NK/T-cell lymphomas. Among the remaining cases, we identified two cell-of-origin subgroups corresponding to cytotoxic/Th1 (n=19) and Th2 (n=24) signatures. A reproducibility test on 40 cases yielded a 90% concordance between three independent laboratories. This study demonstrates the applicability of a simple gene expression assay for the classification of peripheral T-cell lymphomas. Its applicability to routinely-fixed samples makes it an attractive adjunct in diagnostic practice.
Mots-clé
Aggressive Non-Hodgkin's Lymphoma, Cytogenetics and Molecular Genetics, Immunophenotyping, Peripheral T-cell lymphoma, RT-MLPA
Pubmed
Web of science
Open Access
Oui
Création de la notice
13/09/2019 11:16
Dernière modification de la notice
16/12/2023 7:10
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