Effect of conditioned medium, nutritive elements and mitotic synchronization on the accuracy of the cytogenetic analysis in patients with chronic myeloid leukemia at diagnosis and during alpha-interferon therapy

Détails

ID Serval
serval:BIB_9939B9F44C8B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Effect of conditioned medium, nutritive elements and mitotic synchronization on the accuracy of the cytogenetic analysis in patients with chronic myeloid leukemia at diagnosis and during alpha-interferon therapy
Périodique
Cancer Genetics and Cytogenetics
Auteur⸱e⸱s
Castagne  C., Muhlematter  D., Martinet  D., Jotterand  M.
ISSN
0165-4608 (Print)
Statut éditorial
Publié
Date de publication
03/1999
Volume
109
Numéro
2
Pages
166-71
Notes
Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar
Résumé
To improve the yield of the cytogenetic analysis in patients with CML at presentation and during alpha-interferon therapy, three culture conditions for bone marrow or peripheral blood cells were tested in parallel. The effects of 5637 conditioned medium (CM), nutritive elements (NE), and methotrexate (MTX) cell synchronization were investigated in 10 Ph-positive (Ph+) CML patients at diagnosis (group 1), and in 13 Ph+ CML patients receiving treatment with alpha-interferon (group 2). In the presence of 5637 CM and NE with or without MTX, the mitotic index values were significantly improved in both groups. In group 2, the morphological index was significantly increased when using 5637 NE, and percentages of abnormal cells did not differ in 5637 NE and 5637 NE MTX compared to the control condition. Although cessation of interferon administration before sampling may improve the yield of the technique, it does not seem necessary when using 5637 CM and NE. The variability of the response of leukemic cells to different culture conditions further supports the recommendation that, in addition to the control condition, supplementations with 5637 CM and NE with or without cell synchronization be used in parallel in all CML patients. Results suggest that, when the number of cells available is not sufficient for several cultures, 5637 NE with or without MTX should replace the control condition.
Mots-clé
Antimetabolites, Antineoplastic/pharmacology Antineoplastic Agents/*therapeutic use Bone Marrow/drug effects Cell Division/drug effects Culture Media Culture Media, Conditioned/*pharmacology Cytogenetics/methods Humans Interferon-alpha/*therapeutic use Leukemia, Myeloid, Chronic/*drug therapy/*genetics/pathology Leukemia, Myeloid, Philadelphia-Positive/drug therapy/genetics/pathology Methotrexate/pharmacology *Mitotic Index Tumor Cells, Cultured/drug effects
Pubmed
Web of science
Création de la notice
25/01/2008 14:18
Dernière modification de la notice
20/08/2019 15:00
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