Interaction between neuropeptide Y (NPY) and brain-derived neurotrophic factor in NPY-mediated neuroprotection against excitotoxicity : a role for microglia

Détails

ID Serval
serval:BIB_9934EA19FBBB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Interaction between neuropeptide Y (NPY) and brain-derived neurotrophic factor in NPY-mediated neuroprotection against excitotoxicity : a role for microglia
Périodique
European Journal of Neuroscience
Auteur⸱e⸱s
Xapelli S., Bernardino L., Ferreira R., Grade S., Silva A.P., Salgado J.R., Cavadas C., Grouzmann E., Poulsen F.R., Jakobsen B., Oliveira C.R., Zimmer J., Malva J.O.
ISSN
0953-816X
Statut éditorial
Publié
Date de publication
2008
Peer-reviewed
Oui
Volume
27
Numéro
8
Pages
2089-2102
Langue
anglais
Résumé
The neuroprotective effect of neuropeptide Y (NPY) receptor activation was investigated in organotypic mouse hippocampal slice cultures exposed to the glutamate receptor agonist alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA). Exposure of 2-week-old slice cultures, derived from 7-day-old C57BL/6 mice, to 8 microm AMPA, for 24 h, induced degeneration of CA1 and CA3 pyramidal cells, as measured by cellular uptake of propidium iodide (PI). A significant neuroprotection, with a reduction of PI uptake in CA1 and CA3 pyramidal cell layers, was observed after incubation with a Y(2) receptor agonist [NPY(13-36), 300 nm]. This effect was sensitive to the presence of the selective Y(2) receptor antagonist (BIIE0246, 1 microm), but was not affected by addition of TrkB-Fc or by a neutralizing antibody against brain-derived neurotrophic factor (BDNF). Moreover, addition of a Y(1) receptor antagonist (BIBP3226, 1 microm) or a NPY-neutralizing antibody helped to disclose a neuroprotective role of endogenous NPY in CA1 region. Cultures exposed to 8 microm AMPA for 24 h, displayed, as measured by an enzyme-linked immunosorbent assay, a significant increase in BDNF. In such cultures there was an up-regulation of neuronal TrkB immunoreactivity, as well as the presence of BDNF-immunoreactive microglial cells at sites of injury. Thus, an increase of AMPA-receptor mediated neurodegeneration, in the mouse hippocampus, was prevented by neuroprotective pathways activated by NPY receptors (Y(1) and Y(2)), which can be affected by BDNF released by microglia and neurons.
Mots-clé
Animals, Brain-Derived Neurotrophic Factor/metabolism, Enzyme-Linked Immunosorbent Assay, Hippocampus/metabolism, Immunohistochemistry, Mice, Mice, Inbred C57BL, Microglia/metabolism, Neurons/metabolism, Neuropeptide Y/metabolism, Organ Culture Techniques, Receptors, AMPA/metabolism, Receptors, Neuropeptide Y/antagonists & inhibitors, Receptors, Neuropeptide Y/metabolism, Reverse Transcriptase Polymerase Chain Reaction
Pubmed
Web of science
Création de la notice
13/10/2009 13:07
Dernière modification de la notice
20/08/2019 15:00
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