Dexamethasone stimulates the biochemical differentiation of fetal forebrain cells in reaggregating cultures.

Détails

ID Serval
serval:BIB_991A9EE0E693
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Dexamethasone stimulates the biochemical differentiation of fetal forebrain cells in reaggregating cultures.
Périodique
Developmental Neuroscience
Auteur⸱e⸱s
Almazan G., Honegger P., Du Pasquier P., Matthieu J.M.
ISSN
0378-5866 (Print)
ISSN-L
0378-5866
Statut éditorial
Publié
Date de publication
1986
Volume
8
Numéro
1
Pages
14-23
Langue
anglais
Résumé
The influence of dexamethasone on the development of neurons and oligodendrocytes was studied in serum-free, aggregating rat brain cell cultures. Synaptogenesis and myelination occur in this culture system. The concentration of myelin basic protein and the activity of 2',3'-cyclic nucleotide 3'-phosphodiesterase were used as oligodendroglia and myelin markers. Choline acetyltransferase and acetylcholinesterase served as neuronal markers, glutamine synthetase reflected astrocyte differentiation, while ornithine decarboxylase served as a general marker for cell growth and maturation. This study showed that dexamethasone stimulated the differentiation of cholinergic neurons and astrocytes. The effect of dexamethasone on oligodendroglial differentiation and myelination depended on the stage of development: during the early phase of myelination dexamethasone had a stimulatory effect, whereas at a later stage it showed a significant inhibition.
Mots-clé
Acetylcholinesterase/metabolism, Animals, Brain/cytology, Brain/drug effects, Cell Aggregation/drug effects, Cells, Cultured, Choline O-Acetyltransferase/metabolism, Dexamethasone/pharmacology, Female, Fetus, Glutamate-Ammonia Ligase/metabolism, Kinetics, Myelin Basic Protein/analysis, Ornithine Decarboxylase/metabolism, Pregnancy, Rats, Rats, Inbred Strains
Pubmed
Web of science
Création de la notice
24/01/2008 14:12
Dernière modification de la notice
20/08/2019 16:00
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