Staurosporine but not chelerythrine inhibits regeneration in hippocampal organotypic cultures.

Détails

ID Serval
serval:BIB_991A3357FB50
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Staurosporine but not chelerythrine inhibits regeneration in hippocampal organotypic cultures.
Périodique
Synapse
Auteur⸱e⸱s
Toni N., Stoppini L., Muller D.
ISSN
0887-4476
Statut éditorial
Publié
Date de publication
1997
Peer-reviewed
Oui
Volume
27
Numéro
3
Pages
199-207
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
A mechanical lesion in hippocampal organotypic cultures is followed by a recovery process involving scar formation, sprouting of fibres and formation of new functional synapses. Here we tested the effect of staurosporine and chelerythrine, two protein kinase C (PKC) inhibitors, on this lesion-induced neurite outgrowth of Shaffer collaterals. At a concentration of 1 microM, staurosporine delayed functional recovery assessed by measuring synaptic field potentials across the lesion, without altering synaptic transmission on nonlesioned cultures. Immunostaining carried out by using antibodies directed against neurofilament proteins showed that there was a marked reduction in the number of regenerating fibres crossing the lesion. In contrast to this, chelerythrine (50 microM) did not prevent functional recovery, although it affected synaptic transmission and plasticity at this concentration. We conclude that the inhibition of sprouting produced by staurosporine is independent of its blockade of PKC-mediated phosphorylation mechanisms.
Mots-clé
Alkaloids, Animals, Animals, Newborn, Benzophenanthridines, Electrophysiology, Enzyme Inhibitors/pharmacology, Hippocampus/drug effects, Hippocampus/enzymology, Immunohistochemistry, Long-Term Potentiation/drug effects, Nerve Fibers/drug effects, Nerve Fibers/ultrastructure, Nerve Regeneration/drug effects, Neurites/drug effects, Neurites/ultrastructure, Neuronal Plasticity/drug effects, Organ Culture Techniques, Phenanthridines/pharmacology, Protein Kinase C/antagonists & inhibitors, Protein Kinase C/metabolism, Rats, Rats, Wistar, Staurosporine/pharmacology
Pubmed
Web of science
Création de la notice
22/01/2010 9:10
Dernière modification de la notice
20/08/2019 16:00
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