Interstitial dendritic cell guidance by haptotactic chemokine gradients.

Détails

ID Serval
serval:BIB_98F5D5BC554D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Interstitial dendritic cell guidance by haptotactic chemokine gradients.
Périodique
Science
Auteur⸱e⸱s
Weber M., Hauschild R., Schwarz J., Moussion C., de Vries I., Legler D.F., Luther S.A., Bollenbach T., Sixt M.
ISSN
1095-9203 (Electronic)
ISSN-L
0036-8075
Statut éditorial
Publié
Date de publication
2013
Volume
339
Numéro
6117
Pages
328-332
Langue
anglais
Résumé
Directional guidance of cells via gradients of chemokines is considered crucial for embryonic development, cancer dissemination, and immune responses. Nevertheless, the concept still lacks direct experimental confirmation in vivo. Here, we identify endogenous gradients of the chemokine CCL21 within mouse skin and show that they guide dendritic cells toward lymphatic vessels. Quantitative imaging reveals depots of CCL21 within lymphatic endothelial cells and steeply decaying gradients within the perilymphatic interstitium. These gradients match the migratory patterns of the dendritic cells, which directionally approach vessels from a distance of up to 90-micrometers. Interstitial CCL21 is immobilized to heparan sulfates, and its experimental delocalization or swamping the endogenous gradients abolishes directed migration. These findings functionally establish the concept of haptotaxis, directed migration along immobilized gradients, in tissues.
Mots-clé
Animals, Chemokine CCL19/metabolism, Chemokine CCL21/chemistry, Chemokine CCL21/immunology, Chemotaxis/immunology, Dendritic Cells/immunology, Heparitin Sulfate/chemistry, Immobilized Proteins/chemistry, Immobilized Proteins/immunology, Lymphatic Vessels/immunology, Mice, Mice, Inbred C57BL, Mice, Mutant Strains, Receptors, CCR7/genetics, Skin/immunology
Pubmed
Web of science
Création de la notice
07/02/2013 8:39
Dernière modification de la notice
20/08/2019 15:00
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